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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 60,62 kDa (predicted molecular weight: 70 kDa).
Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.
Expressed in platelets (at protein level).
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.
Glycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms. Phosphorylated at Ser-611, Thr-613 and Thr-616.
Cell membrane. Endomembrane system. Endosome membrane. Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin.
Solute carrier family 6 (neurotransmitter transporter) member 4 antibody
solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 antibody
Solute carrier family 6 member 4 antibody
Western blot - Anti-Serotonin transporter antibody (ab172884)
All lanes : Anti-Serotonin transporter antibody (ab172884) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Mouse Serotinergic Nucleus Tissue Lysate Lane 2 : Rat Serotinergic Nucleus Tissue Lysate Lane 3 : Mouse Substantia Nigra Tissue Lysate Lane 4 : Rat Substantia Nigra Tissue Lysate Lane 5 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab172884 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Han X et al. Electroacupuncture restores hippocampal synaptic plasticity via modulation of 5-HT receptors in a rat model of depression. Brain Res Bull139:256-262 (2018).
Read more (PubMed: 29524471) »