Key features and details
- Rabbit polyclonal to Serotonin transporter
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-Serotonin transporter antibody
See all Serotonin transporter primary antibodies
DescriptionRabbit polyclonal to Serotonin transporter
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Sheep, Cow, Human, Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Rat Serotonin transporter aa 200-300 conjugated to keyhole limpet haemocyanin.
Database link: P31652
- This antibody gave a positive signal in the following tissue lysates: Mouse Serotinergic Nucleus; Rat Serotinergic Nucleus; Mouse Substantia Nigra; Rat Substantia Nigra; Rat Cerebellum.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab172884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 60,62 kDa (predicted molecular weight: 70 kDa).|
FunctionSerotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.
Tissue specificityExpressed in platelets (at protein level).
Sequence similaritiesBelongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.
modificationsGlycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms.
Phosphorylated at Ser-611, Thr-613 and Thr-616.
Cellular localizationCell membrane. Endomembrane system. Endosome membrane. Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin.
- Information by UniProt
- 5 HTT antibody
- 5 HTTLPR antibody
- 5 hydroxytryptamine (serotonin) transporter antibody
All lanes : Anti-Serotonin transporter antibody (ab172884) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Mouse Serotinergic Nucleus Tissue Lysate
Lane 2 : Rat Serotinergic Nucleus Tissue Lysate
Lane 3 : Mouse Substantia Nigra Tissue Lysate
Lane 4 : Rat Substantia Nigra Tissue Lysate
Lane 5 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 60,62 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab172884 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab172884 has been referenced in 2 publications.
- Han X et al. Electroacupuncture restores hippocampal synaptic plasticity via modulation of 5-HT receptors in a rat model of depression. Brain Res Bull 139:256-262 (2018). WB . PubMed: 29524471
- Lin WH et al. Novel insights into the effect of paroxetine administration in pilocarpine-induced chronic epileptic rats. Mol Med Rep 16:8245-8252 (2017). PubMed: 28983622