Overview

  • Product name
    Anti-Serotonin transporter antibody
    See all Serotonin transporter primary antibodies
  • Description
    Rabbit polyclonal to Serotonin transporter
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Sheep, Cow, Human, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Rat Serotonin transporter aa 200-300 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P31652

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse Serotinergic Nucleus; Rat Serotinergic Nucleus; Mouse Substantia Nigra; Rat Substantia Nigra; Rat Cerebellum.

Properties

Applications

Our Abpromise guarantee covers the use of ab172884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60,62 kDa (predicted molecular weight: 70 kDa).

Target

  • Function
    Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.
  • Tissue specificity
    Expressed in platelets (at protein level).
  • Sequence similarities
    Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.
  • Post-translational
    modifications
    Glycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms.
    Phosphorylated at Ser-611, Thr-613 and Thr-616.
  • Cellular localization
    Cell membrane. Endomembrane system. Endosome membrane. Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 HTT antibody
    • 5 HTTLPR antibody
    • 5 hydroxytryptamine (serotonin) transporter antibody
    • 5 hydroxytryptamine transporter antibody
    • 5HT transporter antibody
    • 5HTT antibody
    • hSERT antibody
    • HTT antibody
    • Na+/Cl- dependent serotonin transporter antibody
    • OCD1 antibody
    • SC6A4_HUMAN antibody
    • Serotonin transporter 1 antibody
    • SERT antibody
    • SERT1 antibody
    • Slc6a4 antibody
    • Sodium dependent serotonin transporter antibody
    • Sodium-dependent serotonin transporter antibody
    • Solute carrier family 6 (neurotransmitter transporter) member 4 antibody
    • solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 antibody
    • Solute carrier family 6 member 4 antibody
    see all

Images

  • All lanes : Anti-Serotonin transporter antibody (ab172884) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : Mouse Serotinergic Nucleus Tissue Lysate
    Lane 2 : Rat Serotinergic Nucleus Tissue Lysate
    Lane 3 : Mouse Substantia Nigra Tissue Lysate
    Lane 4 : Rat Substantia Nigra Tissue Lysate
    Lane 5 : Cerebellum Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 70 kDa
    Observed band size: 60,62 kDa
    why is the actual band size different from the predicted?


    Exposure time: 16 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab172884 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:
  • Han X  et al. Electroacupuncture restores hippocampal synaptic plasticity via modulation of 5-HT receptors in a rat model of depression. Brain Res Bull 139:256-262 (2018). WB . Read more (PubMed: 29524471) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Dec 14 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Mar 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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