Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12735] to Serotonin transporter
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Serotonin transporter antibody [EPR12735]
See all Serotonin transporter primary antibodies
DescriptionRabbit monoclonal [EPR12735] to Serotonin transporter
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Serotonin transporter aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P31645
- Human fetal liver, placenta, serum, and plasma lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 70 kDa.|
FunctionSerotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.
Tissue specificityExpressed in platelets (at protein level).
Sequence similaritiesBelongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.
modificationsGlycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms.
Phosphorylated at Ser-611, Thr-613 and Thr-616.
Cellular localizationCell membrane. Endomembrane system. Endosome membrane. Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin.
- Information by UniProt
- 5 HTT antibody
- 5 HTTLPR antibody
- 5 hydroxytryptamine (serotonin) transporter antibody
All lanes : Anti-Serotonin transporter antibody [EPR12735] (ab181034) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human placenta lysate
Lane 3 : Human serum lysate
Lane 4 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP-labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 70 kDa
ab181034 has been referenced in 5 publications.
- Ranzil S et al. Disrupted placental serotonin synthetic pathway and increased placental serotonin: Potential implications in the pathogenesis of human fetal growth restriction. Placenta 84:74-83 (2019). PubMed: 31176514
- Cao YN et al. Effect ofLactobacillus rhamnosusGG supernatant on serotonin transporter expression in rats with post-infectious irritable bowel syndrome. World J Gastroenterol 24:338-350 (2018). WB . PubMed: 29391756
- Jackson TC et al. Whole-transcriptome microarray analysis reveals regulation of Rab4 by RBM5 in neurons. Neuroscience 361:93-107 (2017). PubMed: 28818525
- Zhu X et al. Nicotinamide adenine dinucleotide replenishment rescues colon degeneration in aged mice. Signal Transduct Target Ther 2:17017 (2017). PubMed: 29263919
- Yu S et al. Angiogenic microspheres promote neural regeneration and motor function recovery after spinal cord injury in rats. Sci Rep 6:33428 (2016). IHC-P ; Rat . PubMed: 27641997