Recombinant
RabMAb

Recombinant Anti-Serotonin transporter antibody [EPR12735] (ab181034)

Overview

  • Product name

    Anti-Serotonin transporter antibody [EPR12735]
    See all Serotonin transporter primary antibodies
  • Description

    Rabbit monoclonal [EPR12735] to Serotonin transporter
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Serotonin transporter aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P31645

  • Positive control

    • Human fetal liver, placenta, serum, and plasma lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 70 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.
    • Tissue specificity

      Expressed in platelets (at protein level).
    • Sequence similarities

      Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.
    • Post-translational
      modifications

      Glycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms.
      Phosphorylated at Ser-611, Thr-613 and Thr-616.
    • Cellular localization

      Cell membrane. Endomembrane system. Endosome membrane. Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin.
    • Information by UniProt
    • Database links

    • Alternative names

      • 5 HTT antibody
      • 5 HTTLPR antibody
      • 5 hydroxytryptamine (serotonin) transporter antibody
      • 5 hydroxytryptamine transporter antibody
      • 5HT transporter antibody
      • 5HTT antibody
      • hSERT antibody
      • HTT antibody
      • Na+/Cl- dependent serotonin transporter antibody
      • OCD1 antibody
      • SC6A4_HUMAN antibody
      • Serotonin transporter 1 antibody
      • SERT antibody
      • SERT1 antibody
      • Slc6a4 antibody
      • Sodium dependent serotonin transporter antibody
      • Sodium-dependent serotonin transporter antibody
      • Solute carrier family 6 (neurotransmitter transporter) member 4 antibody
      • solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 antibody
      • Solute carrier family 6 member 4 antibody
      see all

    Images

    • All lanes : Anti-Serotonin transporter antibody [EPR12735] (ab181034) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human placenta lysate
      Lane 3 : Human serum lysate
      Lane 4 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP-labeled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 70 kDa

    References

    This product has been referenced in:

    • Cao YN  et al. Effect ofLactobacillus rhamnosusGG supernatant on serotonin transporter expression in rats with post-infectious irritable bowel syndrome. World J Gastroenterol 24:338-350 (2018). WB . Read more (PubMed: 29391756) »
    • Jackson TC  et al. Whole-transcriptome microarray analysis reveals regulation of Rab4 by RBM5 in neurons. Neuroscience 361:93-107 (2017). Read more (PubMed: 28818525) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 15 2015

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 14 2015

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    28 µg
    Specification
    Intestinal tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Aug 13 2015

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (intestinal tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    28 µg
    Specification
    intestinal tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jul 24 2015

    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    28 µg
    Specification
    Intestinal tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Mr. Julien Chevalier

    Verified customer

    Submitted Jul 24 2015

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