Anti-Serotonin transporter (phospho T276) antibody (ab194262)
Key features and details
- Rabbit polyclonal to Serotonin transporter (phospho T276)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Serotonin transporter (phospho T276) antibody
See all Serotonin transporter primary antibodies -
Description
Rabbit polyclonal to Serotonin transporter (phospho T276) -
Host species
Rabbit -
Specificity
ab194262 is specific for Serotonin transporter phosphorylated at Thr276. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to generate the antibody but not by the corresponding dephospho-peptide. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Sheep, Cow, Human, Non human primates -
Immunogen
Synthetic peptide corresponding to Rat Serotonin transporter (phospho T276).
Database link: P31652 -
Positive control
- Rat mid brain membrane lysate.
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituents: 50% Glycerol, 0.24% HEPES, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab194262 was prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho- peptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 70 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 70 kDa. |
Target
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Function
Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner. -
Tissue specificity
Expressed in platelets (at protein level). -
Sequence similarities
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily. -
Post-translational
modificationsGlycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms.
Phosphorylated at Ser-611, Thr-613 and Thr-616. -
Cellular localization
Cell membrane. Endomembrane system. Endosome membrane. Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin. - Information by UniProt
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Database links
- Entrez Gene: 282365 Cow
- Entrez Gene: 6532 Human
- Entrez Gene: 15567 Mouse
- Entrez Gene: 25553 Rat
- Entrez Gene: 443491 Sheep
- Omim: 182138 Human
- SwissProt: Q9XT49 Cow
- SwissProt: P31645 Human
see all -
Alternative names
- 5 HTT antibody
- 5 HTTLPR antibody
- 5 hydroxytryptamine (serotonin) transporter antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab194262 has not yet been referenced specifically in any publications.