Overview

  • Product name

  • Description

    Rabbit polyclonal to SERP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 7-56 (IRMANEKHSK NITQRGNVAK TSRNAPEEKA SVGPWLLALF IFVVCGSAIF) of Human SERP1 (NP_055260.1).

  • Positive control

    • MCF7 cell line lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab130974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 7 kDa.

Target

  • Function

    Interacts with target proteins during their translocation into the lumen of the endoplasmic reticulum. Protects unfolded target proteins against degradation during ER stress. May facilitate glycosylation of target proteins after termination of ER stress. May modulate the use of N-glycosylation sites on target proteins.
  • Sequence similarities

    Belongs to the RAMP4 family.
  • Cellular localization

    Membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • D3Ucla1 antibody
    • Ramp4 antibody
    • Ribosome associated membrane protein 4 antibody
    • Ribosome attached membrane protein 4 antibody
    • Ribosome-attached membrane protein 4 antibody
    • Serp1 antibody
    • SERP1_HUMAN antibody
    • Stress associated endoplasmic reticulum protein 1 antibody
    • Stress-associated endoplasmic reticulum protein 1 antibody
    see all

Images

  • Anti-SERP1 antibody (ab130974) at 1 µg/ml + MCF7 cell line lysate at 10 µg

    Secondary
    Goat anti-Rabbit IgG HRP

    Predicted band size: 7 kDa



    Gel concentration: 10-20%

References

This product has been referenced in:

  • Ma Q  et al. SERP1 is a novel marker of poor prognosis in pancreatic ductal adenocarcinoma patients via anti-apoptosis and regulating SRPRB/NF-?B axis. Int J Oncol 51:1104-1114 (2017). Read more (PubMed: 28902358) »
See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Lymphocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Treatment
4ug/mL tunicamycin 8 hours
Specification
Lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 27 2017

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