Overview

  • Product name

  • Description

    Mouse polyclonal to Serpin A5
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Serpin A5 aa 1-406.

  • Positive control

    • Human spleen tissue lysate; Serpin A5 transfected 293T cell lysate.
  • General notes

    Previously labelled as Protein C inhibitor. 

Properties

Applications

Our Abpromise guarantee covers the use of ab67368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
    Detection Pair.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Inhibits activated protein C as well as plasminogen activators.
    • Tissue specificity

      Expressed by the liver and secreted in plasma.
    • Sequence similarities

      Belongs to the serpin family.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Acrosomal serine protease inhibitor antibody
      • IPSP antibody
      • IPSP_HUMAN antibody
      • PAI 3 antibody
      • PAI-3 antibody
      • PAI3 antibody
      • PCI antibody
      • PCI-B antibody
      • PLANH 3 antibody
      • PLANH3 antibody
      • Plasma serine protease inhibitor antibody
      • Plasminogen activator inhibitor 3 antibody
      • Plasminogen activator inhibitor III antibody
      • PROCI antibody
      • Protein C inhibitor antibody
      • Serpin A5 antibody
      • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 5 antibody
      • Serpin peptidase inhibitor clade A member 5 antibody
      • Serpina5 antibody
      see all

    Images

    • Anti-Serpin A5 antibody (ab67368) at 1/500 dilution + Human spleen tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size: 46 kDa
      Observed band size: 46 kDa

    • All lanes : Anti-Serpin A5 antibody (ab67368) at 1/500 dilution

      Lane 1 : Serpin A5 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size: 46 kDa
      Observed band size: 37 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Do M  et al. Quantitative proteomic analysis of pancreatic cyst fluid proteins associated with malignancy in intraductal papillary mucinous neoplasms. Clin Proteomics 15:17 (2018). Read more (PubMed: 29713252) »
    • Lawlor K  et al. Pathway-based biomarker search by high-throughput proteomics profiling of secretomes. J Proteome Res 8:1489-503 (2009). Read more (PubMed: 19199430) »
    See all 2 Publications for this product

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