Recombinant
RabMAb

Anti-SERPINB1 antibody [EPR13305(B)] (ab181084)

Overview

  • Product name
    Anti-SERPINB1 antibody [EPR13305(B)]
    See all SERPINB1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13305(B)] to SERPINB1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SERPINB1 aa 250-350. The exact sequence is proprietary.
    Database link: P30740

  • Positive control
    • Human fetal lung tissue lysate; MCF7, HeLa, HepG2 whole cell lysate; HeLa cells; HepG2 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
ICC/IF 1/50 - 1/100.
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3.
  • Sequence similarities
    Belongs to the serpin family. Ov-serpin subfamily.
  • Domain
    Reactive bond 1 is specific for reaction with chymotrypsin-like protease such as cathepsin G, chymotrypsin or chymase, while reactive bond 2 is specific for reaction with elastase-like protease such as neutrophyl elastase, proteinase-3, pancreatic elastase or PSA.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anti elastase antibody
    • EI antibody
    • ELANH2 antibody
    • Epididymis luminal protein 57 antibody
    • HEL57 antibody
    • ILEU_HUMAN antibody
    • LEI antibody
    • Leukocyte elastase inhibitor antibody
    • M/NEI antibody
    • MNEI antibody
    • Monocyte/neutrophil elastase inhibitor antibody
    • Peptidase inhibitor 2 antibody
    • Pi-2 antibody
    • PI2 antibody
    • Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 1 antibody
    • Serpin B1 antibody
    • Serpin peptidase inhibitor clade B (ovalbumin) member 1 antibody
    • serpinb1 antibody
    see all

Images

  • All lanes : Anti-SERPINB1 antibody [EPR13305(B)] (ab181084) at 1/1000 dilution

    Lane 1 : Human fetal lung tissue lysate
    Lane 2 : MCF7 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 43 kDa

  • Anti-SERPINB1 antibody [EPR13305(B)] (ab181084) at 1/1000 dilution + HepG2 whole cell lysate at 20 µg

    Predicted band size: 43 kDa

  • Flow cytometric analysis of HepG2 cells fixed with 2% paraformaldehyde labeling SERPINB1 with ab181084 at 1/70 dilution (red), compared to a rabbit IgG negative control (green).

  • Immunofluorescent analysis of HeLa cells labeling SERPINB1 with ab181084 at a 1/100 dilution. Primary incubation was followed by secondary antibody labeling with Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • ab181084 staining SERPINB1 in human colorectal cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris/HCl pH 9.0. Samples were incubated with primary antibody (1/500) for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

References

ab181084 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: HIER Tris/HCL pH 9.0
Sample
Human Tissue sections (Human Colorectal Cancer)
Specification
Human Colorectal Cancer
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
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Submitted Aug 08 2014

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