Western blot abreview for Anti-SERPINB13 antibody

Average
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse serpin B13 overexpression in 293T)
Gel Running Conditions
Reduced Denaturing (NuPAGE 4-12% Bis-Tris Gel)
Loading amount
10 µg
Specification
mouse serpin B13 overexpression in 293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in PBST
Dilution
1/1000

Secondary antibody

Dilution
1/20000
Name
Non-Abcam antibody was used: anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
SuperSignal™ West Femto Maximum Sensitiv
Exposure
10 second(s)
Bands
Specific: about 44 kDa
Positive control
Purified human serpin B13 protein

Dr. Chi-Wen Lo

Verified customer

Submitted Feb 03 2017

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