Key features and details
- Mouse monoclonal [OTI2B6] to SERPINB13
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-SERPINB13 antibody [OTI2B6]
DescriptionMouse monoclonal [OTI2B6] to SERPINB13
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human SERPINB13 aa 1-391. Produced in HEK-293T cells (NP_036529).
MDSLGAVSTRLGFDLFKELKKTNDGNIFFSPVGILTAIGMVLLGTRGATA SQLEEVFHSEKETKSSRIKAEEKEVIENTEAVHQQFQKFLTEISKLTNDY ELNITNRLFGEKTYLFLQKYLDYVEKYYHASLEPVDFVNAADESRKKINS WVESKTNEKIKDLFPDGSISSSTKLVLVNMVYFKGQWDREFKKENTKEEK FWMNKSTSKSVQMMTQSHSFSFTFLEDLQAKILGIPYKNNDLSMFVLLPN DIDGLEKIIDKISPEKLVEWTSPGHMEERKVNLHLPRFEVEDGYDLEAVL AAMGMGDAFSEHKADYSGMSSGSGLYAQKFLHSSFVAVTEEGTEAAAATG IGFTVTSAPGHENVHCNHPFLFFIRHNESNSILFFGRFSSP
Database link: Q9UIV8
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY SERPINB13 cDNA. IHC-P: Human breast adenocarcinoma, kidney, liver and bladder carcinoma tissues.
Clone OTI2B6 (formerly 2B6).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000. Predicted molecular weight: 44 kDa.|
FunctionMay play a role in the proliferation or differentiation of keratinocytes.
Tissue specificitySkin specific.
Sequence similaritiesBelongs to the serpin family. Ov-serpin subfamily.
- Information by UniProt
- HaCaT UV repressible serpin antibody
- HaCaT UV-repressible serpin antibody
- Headpin antibody
All lanes : Anti-SERPINB13 antibody [OTI2B6] (ab236412) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY SERPINB13 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 44 kDa
Paraffin-embedded human breast adenocarcinoma tissue stained for SERPINB13 using ab236412 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for SERPINB13 using ab236412 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for SERPINB13 using ab236412 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human bladder carcinoma tissue stained for SERPINB13 using ab236412 at 1/150 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236412 has not yet been referenced specifically in any publications.