Overview

  • Product name

    Anti-SERPINB1/PI2 antibody
    See all SERPINB1/PI2 primary antibodies
  • Description

    Goat polyclonal to SERPINB1/PI2
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chimpanzee, Monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human SERPINB1/PI2 aa 130-143 (internal sequence) (Cysteine residue). (NP_109591.1).
    Sequence:

    KTINQWVKGQTEGK


    Database link: P30740

  • Positive control

    • Human spleen neutrophils; Human placenta lysate.
  • General notes

     This product was previously labelled as SERPINB1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab195969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 42-45 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3.
  • Sequence similarities

    Belongs to the serpin family. Ov-serpin subfamily.
  • Domain

    Reactive bond 1 is specific for reaction with chymotrypsin-like protease such as cathepsin G, chymotrypsin or chymase, while reactive bond 2 is specific for reaction with elastase-like protease such as neutrophyl elastase, proteinase-3, pancreatic elastase or PSA.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Anti elastase antibody
    • EI antibody
    • ELANH2 antibody
    • Epididymis luminal protein 57 antibody
    • HEL57 antibody
    • ILEU_HUMAN antibody
    • LEI antibody
    • Leukocyte elastase inhibitor antibody
    • M/NEI antibody
    • MNEI antibody
    • Monocyte/neutrophil elastase inhibitor antibody
    • Peptidase inhibitor 2 antibody
    • Pi-2 antibody
    • PI2 antibody
    • Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 1 antibody
    • Serpin B1 antibody
    • Serpin peptidase inhibitor clade B (ovalbumin) member 1 antibody
    • serpinb1 antibody
    see all

Images

  • All lanes : Anti-SERPINB1/PI2 antibody (ab195969) at 0.5 µg/ml

    Lane 1 : Human placenta lysate (in RIPA buffer)
    Lane 2 : Human placenta lysate (in RIPA buffer) with immunizing peptide

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 43 kDa
    Observed band size: 42-45 kDa
    why is the actual band size different from the predicted?



    The fainter 45 kDa band likely represents the acetylated form. Both bands are blocked by the immunizing peptide.

     

    Primary incubation was 1 hour.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling SERPINB1/PI2 with ab195969 at 5 µg/ml.

References

ab195969 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab195969.
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