Product nameAnti-SERPINB1/PI2 antibody
See all SERPINB1/PI2 primary antibodies
DescriptionRabbit polyclonal to SERPINB1/PI2
Tested applicationsSuitable for: WB, ICC/IF, IP, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human SERPINB1/PI2 aa 1-379.
MEQLSSANTRFALDLFLALSENNPAGNIFISPFSISSAMAMVFLGTRGNT AAQLSKTFHFNTVEEVHSRFQSLNADINKRGASYILKLANRLYGEKTYNF LPEFLVSTQKTYGADLASVDFQHASEDARKTINQWVKGQTEGKIPELLAS GMVDNMTKLVLVNAIYFKGNWKDKFMKEATTNAPFRLNKKDRKTVKMMYQ KKKFAYGYIEDLKCRVLELPYQGEELSMVILLPDDIEDESTGLKKIEEQL TLEKLHEWTKPENLDFIEVNVSLPRFKLEESYTLNSDLARLGVQDLFNSS KADLSGMSGARDIFISKIVHKSFVEVNEEGTEAAAATAGIATFCMLMPEE NFTADHPFLFFIRHNSSGSILFLGRFSSP
Database link: P30740
- WB: HepG2, HeLa, A549 and HEK-293T whole cell lysates. IHC-P: Human skin and colon cancer tissue. IC/IF: HeLa cells. IP: HepG2 whole cell lysate.
This product was previously labelled as SERPINB1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 43 kDa.|
|ICC/IF||1/50 - 1/200.|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
FunctionRegulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3.
Sequence similaritiesBelongs to the serpin family. Ov-serpin subfamily.
DomainReactive bond 1 is specific for reaction with chymotrypsin-like protease such as cathepsin G, chymotrypsin or chymase, while reactive bond 2 is specific for reaction with elastase-like protease such as neutrophyl elastase, proteinase-3, pancreatic elastase or PSA.
- Information by UniProt
- Anti elastase antibody
- EI antibody
- ELANH2 antibody
All lanes : Anti-SERPINB1/PI2 antibody (ab236890) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 43 kDa
Paraffin-embedded human skin tissue stained for SERPINB1/PI2 using ab236890 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human colon cancer tissue stained for SERPINB1/PI2 using ab236890 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SERPINB1/PI2(green) using ab236890 at 1/66 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counter stain is DAPI (blue).
The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.
SERPINB1/PI2 was immunoprecipitated from 0.5 mg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab236890 at 1/200 dilution.
Lane 1: Control rabbit IgG IP (1 μg) in HepG2 whole cell lysate.
Lane 2: ab236890 IP in HepG2 whole cell lysate.
Lane 3: HepG2 whole cell lysate 20 μg (Input).
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
ab236890 has not yet been referenced specifically in any publications.