Recombinant
RabMAb

Recombinant Anti-SERPINB1/PI2 antibody [EPR13299] - Membrane Vesicle Marker (ab190357)

Overview

  • Product name

    Anti-SERPINB1/PI2 antibody [EPR13299] - Membrane Vesicle Marker
    See all SERPINB1/PI2 primary antibodies
  • Description

    Rabbit monoclonal [EPR13299] to SERPINB1/PI2 - Membrane Vesicle Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SERPINB1/PI2 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P30740

  • Positive control

    • Human fetal kidney, Human fetal lung, Human fetal liver and Human stomach lysates; HepG2, A549 and MCF7 cell lysates; U937 cells.
  • General notes

     

     

     This product was previously labelled as SERPINB1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab190357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/210.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/100 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3.
  • Sequence similarities

    Belongs to the serpin family. Ov-serpin subfamily.
  • Domain

    Reactive bond 1 is specific for reaction with chymotrypsin-like protease such as cathepsin G, chymotrypsin or chymase, while reactive bond 2 is specific for reaction with elastase-like protease such as neutrophyl elastase, proteinase-3, pancreatic elastase or PSA.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Anti elastase antibody
    • EI antibody
    • ELANH2 antibody
    • Epididymis luminal protein 57 antibody
    • HEL57 antibody
    • ILEU_HUMAN antibody
    • LEI antibody
    • Leukocyte elastase inhibitor antibody
    • M/NEI antibody
    • MNEI antibody
    • Monocyte/neutrophil elastase inhibitor antibody
    • Peptidase inhibitor 2 antibody
    • Pi-2 antibody
    • PI2 antibody
    • Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 1 antibody
    • Serpin B1 antibody
    • Serpin peptidase inhibitor clade B (ovalbumin) member 1 antibody
    • serpinb1 antibody
    see all

Images

  • All lanes : Anti-SERPINB1/PI2 antibody [EPR13299] - Membrane Vesicle Marker (ab190357) at 1/5000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

  • All lanes : Anti-SERPINB1/PI2 antibody [EPR13299] - Membrane Vesicle Marker (ab190357) at 1/5000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal lung lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : Human stomach lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

  • Flow Cytometrical analysis of U937 cells labeling SERPINB1/PI2 with ab190357 at 1/210 (red) compared to a Rabbit monoclonal IgG as an isotope control (green). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab190357 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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