RecombinantRabMAb

Recombinant Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)

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Western blot - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)
  • Flow Cytometry (Intracellular) - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)
  • Immunocytochemistry - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)
  • Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13305(B)] to SERPINB1/PI2
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-SERPINB1/PI2 antibody [EPR13305(B)]
    See all SERPINB1/PI2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13305(B)] to SERPINB1/PI2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: HeLa cells; Flow Cyt (intra): HeLa cells. WB: HeLa and human lung cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  For unpurified use at 1/70 dilution.
WB
1/2000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

For unpurified use at 1/100 - 1/1000 dilution
ICC/IF
1/50 - 1/100.
Notes
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  For unpurified use at 1/70 dilution.
WB
1/2000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

For unpurified use at 1/100 - 1/1000 dilution
ICC/IF
1/50 - 1/100.

Target

  • Function

    Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3.

  • Sequence similarities

    Belongs to the serpin family. Ov-serpin subfamily.

  • Domain

    Reactive bond 1 is specific for reaction with chymotrypsin-like protease such as cathepsin G, chymotrypsin or chymase, while reactive bond 2 is specific for reaction with elastase-like protease such as neutrophyl elastase, proteinase-3, pancreatic elastase or PSA.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Anti elastase antibody
      • EI antibody
      • ELANH2 antibody
      • Epididymis luminal protein 57 antibody
      • HEL57 antibody
      • ILEU_HUMAN antibody
      • LEI antibody
      • Leukocyte elastase inhibitor antibody
      • M/NEI antibody
      • MNEI antibody
      • Monocyte/neutrophil elastase inhibitor antibody
      • Peptidase inhibitor 2 antibody
      • Pi-2 antibody
      • PI2 antibody
      • Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 1 antibody
      • Serpin B1 antibody
      • Serpin peptidase inhibitor clade B (ovalbumin) member 1 antibody
      • serpinb1 antibody
      see all

    Images

    • Western blot - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)
      Western blot - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)
      Lane 1 : Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084) at 1/2000 dilution
      Lane 2 : Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084) at 1/2000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
      Lane 2 : Human lung lysate

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 43 kDa



      We are unsure how to define the extra bands.

    • Flow Cytometry (Intracellular) - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)
      Flow Cytometry (Intracellular) - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)

      Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SERPINB1/PI2 with Purified ab181084 at 1/50 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

       

    • Immunocytochemistry - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)
      Immunocytochemistry - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)
      Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SERPINB1/PI2 with Purified ab181084 at 1/50 dilution (8.9 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)
      Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)

    References (1)

    Publishing research using ab181084? Please let us know so that we can cite the reference in this datasheet.

    ab181084 has been referenced in 1 publication.

    • Liang X  et al. Forkhead box protein O1 (FoxO1) /SERPINB1 ameliorates ROS production in diabetic nephropathy. Food Sci Nutr 9:44-51 (2021). PubMed: 33473269

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SERPINB1 antibody [EPR13305(B)]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: HIER Tris/HCL pH 9.0
    Sample
    Human Tissue sections (Human Colorectal Cancer)
    Specification
    Human Colorectal Cancer
    Permeabilization
    Yes - 0.2% Triton X-100 and PBS
    Fixative
    Formaldehyde
    Read More

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    Verified customer

    Submitted Aug 08 2014

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