Recombinant Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)
![Western blot - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)](https://www.abcam.com/ps/products/181/ab181084/Images/ab181084-449561-anti-serpinb1-pi2-antibody-epr13305-b-westernblot-human.jpg "Western blot - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13305(B)] to SERPINB1/PI2
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SERPINB1/PI2 antibody [EPR13305(B)]
See all SERPINB1/PI2 primary antibodies -
Description
Rabbit monoclonal [EPR13305(B)] to SERPINB1/PI2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: HeLa cells; Flow Cyt (intra): HeLa cells. WB: HeLa and human lung cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13305(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/70 dilution. |
|
| WB |
1/2000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
For unpurified use at 1/100 - 1/1000 dilution |
|
| ICC/IF |
1/50 - 1/100.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/70 dilution. |
|
WB
1/2000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). For unpurified use at 1/100 - 1/1000 dilution |
|
ICC/IF
1/50 - 1/100. |
Target
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Function
Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. -
Sequence similarities
Belongs to the serpin family. Ov-serpin subfamily. -
Domain
Reactive bond 1 is specific for reaction with chymotrypsin-like protease such as cathepsin G, chymotrypsin or chymase, while reactive bond 2 is specific for reaction with elastase-like protease such as neutrophyl elastase, proteinase-3, pancreatic elastase or PSA. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1992 Human
- Omim: 130135 Human
- SwissProt: P30740 Human
- Unigene: 381167 Human
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Alternative names
- Anti elastase antibody
- EI antibody
- ELANH2 antibody
see all
Images
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Western blot - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)Lane 1 : Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084) at 1/2000 dilution
Lane 2 : Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084) at 1/2000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2 : Human lung lysate
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 43 kDaWe are unsure how to define the extra bands.
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![Flow Cytometry (Intracellular) - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)](https://www.abcam.com/ps/products/181/ab181084/Images/ab181084-8-anti-serpinb1-pi2-antibody-epr13305-b-flowcytometry-hela-human.jpg)
Flow Cytometry (Intracellular) - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SERPINB1/PI2 with Purified ab181084 at 1/50 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunocytochemistry - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)](https://www.abcam.com/ps/products/181/ab181084/Images/ab181084-7-anti-serpinb1-pi2-antibody-epr13305-b-immunocytochemistry-hela-human.jpg)
Immunocytochemistry - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SERPINB1/PI2 with Purified ab181084 at 1/50 dilution (8.9 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)](https://www.abcam.com/ps/products/181/ab181084/Images/ab181084-6-benefits-of-recombinant-antibodies.png)
Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab181084 has been referenced in 1 publication.
- Liang X et al. Forkhead box protein O1 (FoxO1) /SERPINB1 ameliorates ROS production in diabetic nephropathy. Food Sci Nutr 9:44-51 (2021). PubMed: 33473269
