Recombinant Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)
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- References
- Protocols
Overview
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Product name
Anti-SERPINB1/PI2 antibody [EPR13305(B)]
See all SERPINB1/PI2 primary antibodies -
Description
Rabbit monoclonal [EPR13305(B)] to SERPINB1/PI2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SERPINB1/PI2 aa 250-350. The exact sequence is proprietary.
Database link: P30740 -
Positive control
- Human fetal lung tissue lysate; MCF7, HeLa, HepG2 whole cell lysate; HeLa cells; HepG2 cells
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General notes
This product was previously labelled as SERPINB1
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13305(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab181084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). | |
| ICC/IF | 1/50 - 1/100. | |
| Flow Cyt | 1/70. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| IHC-P | 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. -
Sequence similarities
Belongs to the serpin family. Ov-serpin subfamily. -
Domain
Reactive bond 1 is specific for reaction with chymotrypsin-like protease such as cathepsin G, chymotrypsin or chymase, while reactive bond 2 is specific for reaction with elastase-like protease such as neutrophyl elastase, proteinase-3, pancreatic elastase or PSA. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1992 Human
- Omim: 130135 Human
- SwissProt: P30740 Human
- Unigene: 381167 Human
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Alternative names
- Anti elastase antibody
- EI antibody
- ELANH2 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)](https://www.abcam.com/ps/products/181/ab181084/Images/ab181084-230991-anti-serpinb1-antibody-epr13305b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)This image is courtesy of an anonymous Abreviewab181084 staining SERPINB1/PI2 in human colorectal cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris/HCl pH 9.0. Samples were incubated with primary antibody (1/500) for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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![Western blot - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)](https://www.abcam.com/ps/products/181/ab181084/Images/ab181084-214773-anti-serpinb1-antibody-epr13305b-western-blot.jpg)
Western blot - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)All lanes : Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084) at 1/1000 dilution
Lane 1 : Human fetal lung tissue lysate
Lane 2 : MCF7 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 43 kDa -
![Western blot - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)](https://www.abcam.com/ps/products/181/ab181084/Images/ab181084-214774-anti-serpinb1-antibody-epr13305b-western-blot.jpg)
Western blot - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084) at 1/1000 dilution + HepG2 whole cell lysate at 20 µg
Predicted band size: 43 kDa -
![Flow Cytometry - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)](https://www.abcam.com/ps/products/181/ab181084/Images/ab181084-214777-anti-serpinb1-antibody-epr13305b-flow-cytometry.jpg)
Flow Cytometry - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)Flow cytometric analysis of HepG2 cells fixed with 2% paraformaldehyde labeling SERPINB1/PI2 with ab181084 at 1/70 dilution (red), compared to a rabbit IgG negative control (green).
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![Immunocytochemistry/ Immunofluorescence - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)](https://www.abcam.com/ps/products/181/ab181084/Images/ab181084-214775-anti-serpinb1-antibody-epr13305b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SERPINB1/PI2 antibody [EPR13305(B)] (ab181084)Immunofluorescent analysis of HeLa cells labeling SERPINB1/PI2 with ab181084 at a 1/100 dilution. Primary incubation was followed by secondary antibody labeling with Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Protocols
Datasheets and documents
References
ab181084 has not yet been referenced specifically in any publications.
