Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (ab195969)
Key features and details
- Goat polyclonal to SERPINB1/PI2 - Membrane Vesicle Marker
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker
See all SERPINB1/PI2 primary antibodies -
Description
Goat polyclonal to SERPINB1/PI2 - Membrane Vesicle Marker -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chimpanzee, Monkey, Gorilla
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Immunogen
Synthetic peptide corresponding to Human SERPINB1/PI2 aa 130-143 (internal sequence) (Cysteine residue). (NP_109591.1).
Sequence:KTINQWVKGQTEGK
Database link: P30740 -
Positive control
- Human spleen neutrophils; Human placenta lysate.
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General notes
This product was previously labelled as SERPINB1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab195969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 42-45 kDa (predicted molecular weight: 43 kDa). |
Target
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Function
Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. -
Sequence similarities
Belongs to the serpin family. Ov-serpin subfamily. -
Domain
Reactive bond 1 is specific for reaction with chymotrypsin-like protease such as cathepsin G, chymotrypsin or chymase, while reactive bond 2 is specific for reaction with elastase-like protease such as neutrophyl elastase, proteinase-3, pancreatic elastase or PSA. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 471841 Chimpanzee
- Entrez Gene: 100049797 Horse
- Entrez Gene: 1992 Human
- Omim: 130135 Human
- SwissProt: P05619 Horse
- SwissProt: P30740 Human
- SwissProt: Q9D154 Mouse
- SwissProt: Q4G075 Rat
see all -
Alternative names
- Anti elastase antibody
- EI antibody
- ELANH2 antibody
see all
Images
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Western blot - Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (ab195969)All lanes : Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (ab195969) at 0.5 µg/ml
Lane 1 : Human placenta lysate (in RIPA buffer)
Lane 2 : Human placenta lysate (in RIPA buffer) with immunizing peptide
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 42-45 kDa why is the actual band size different from the predicted?The fainter 45 kDa band likely represents the acetylated form. Both bands are blocked by the immunizing peptide.
Primary incubation was 1 hour.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (ab195969)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling SERPINB1/PI2 with ab195969 at 5 µg/ml.
Protocols
Datasheets and documents
References (0)
ab195969 has not yet been referenced specifically in any publications.
