Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (ab195969)
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Overview
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Product name
Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker
See all SERPINB1/PI2 primary antibodies -
Description
Goat polyclonal to SERPINB1/PI2 - Membrane Vesicle Marker -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chimpanzee, Monkey, Gorilla
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Immunogen
Synthetic peptide corresponding to Human SERPINB1/PI2 aa 130-143 (internal sequence) (Cysteine residue). (NP_109591.1).
Sequence:KTINQWVKGQTEGK
Database link: P30740 -
Positive control
- Human spleen neutrophils; Human placenta lysate.
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General notes
This product was previously labelled as SERPINB1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab195969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 42-45 kDa (predicted molecular weight: 43 kDa). |
Target
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Function
Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. -
Sequence similarities
Belongs to the serpin family. Ov-serpin subfamily. -
Domain
Reactive bond 1 is specific for reaction with chymotrypsin-like protease such as cathepsin G, chymotrypsin or chymase, while reactive bond 2 is specific for reaction with elastase-like protease such as neutrophyl elastase, proteinase-3, pancreatic elastase or PSA. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 471841 Chimpanzee
- Entrez Gene: 100049797 Horse
- Entrez Gene: 1992 Human
- Omim: 130135 Human
- SwissProt: P05619 Horse
- SwissProt: P30740 Human
- SwissProt: Q9D154 Mouse
- SwissProt: Q4G075 Rat
see all -
Alternative names
- Anti elastase antibody
- EI antibody
- ELANH2 antibody
see all
Images
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Western blot - Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (ab195969)All lanes : Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (ab195969) at 0.5 µg/ml
Lane 1 : Human placenta lysate (in RIPA buffer)
Lane 2 : Human placenta lysate (in RIPA buffer) with immunizing peptide
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 42-45 kDa why is the actual band size different from the predicted?The fainter 45 kDa band likely represents the acetylated form. Both bands are blocked by the immunizing peptide.
Primary incubation was 1 hour.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINB1/PI2 antibody - Membrane Vesicle Marker (ab195969)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling SERPINB1/PI2 with ab195969 at 5 µg/ml.
Datasheets and documents
References
ab195969 has not yet been referenced specifically in any publications.
