For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    serpinb2pai-2-antibody-ab47742.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Macrophage / Inflamm.
Share by email

Anti-SerpinB2/PAI-2 antibody (ab47742)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (8)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody (ab47742)
  • Western blot - Anti-SerpinB2/PAI-2 antibody (ab47742)

Key features and details

  • Rabbit polyclonal to SerpinB2/PAI-2
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Western blot
Product image
InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)
Primary
Product image
Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
Primary
Product image
Anti-TIMP2 antibody [3A4] (ab1828)

View more associated products

Overview

  • Product name

    Anti-SerpinB2/PAI-2 antibody
    See all SerpinB2/PAI-2 primary antibodies
  • Description

    Rabbit polyclonal to SerpinB2/PAI-2
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for SerpinB2/PAI-2 (but not the shorter form).

  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human SerpinB2/PAI-2 (N terminal).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Serine protease inhibitors
    • SERPINs

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human SerpinB2/PAI-2 protein (ab69515)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab47742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB (1)
Notes
Application notes
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 46 kDa. Dilution optimised using Chromogenic detection.

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Target

  • Function

    Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.
  • Sequence similarities

    Belongs to the serpin family. Ov-serpin subfamily.
  • Post-translational
    modifications

    The signal sequence is not cleaved.
  • Cellular localization

    Cytoplasm. Secreted > extracellular space.
  • Target information above from: UniProt accession P05120 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5055 Human
    • Omim: 173390 Human
    • SwissProt: P05120 Human
    • Unigene: 594481 Human
    • Alternative names

      • HsT1201 antibody
      • Monocyte Arg serpin antibody
      • Monocyte Arg-serpin antibody
      • Monocyte Arginine-serpin antibody
      • Monocyte-derived plasminogen activator inhibitor antibody
      • PAI antibody
      • PAI-2 antibody
      • PAI2 antibody
      • PAI2_HUMAN antibody
      • Placental plasminogen activator inhibitor antibody
      • PLANH2 antibody
      • Plasminogen activator inhibitor 2 antibody
      • plasminogen activator inhibitor, type 2 antibody
      • plasminogen activator inhibitor, type II (arginine-serpin) antibody
      • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2 antibody
      • Serpin B2 antibody
      • serpin peptidase inhibitor, clade B (ovalbumin), member 2 antibody
      • Serpinb2 antibody
      • Urokinase inhibitor antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody (ab47742)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody (ab47742)
      Ab47742 staining human normal temporal lobe. Staining is localized to the cytoplasm.
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Western blot - Anti-SerpinB2/PAI-2 antibody (ab47742)
      Western blot - Anti-SerpinB2/PAI-2 antibody (ab47742)
      Anti-SerpinB2/PAI-2 antibody (ab47742) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 46 kDa
      Observed band size: 49 kDa why is the actual band size different from the predicted?
      Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 minute


      SerpinB2/PAI-2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    Protocols

    • Recommended WB protocol

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (8)

    Publishing research using ab47742? Please let us know so that we can cite the reference in this datasheet.

    ab47742 has been referenced in 8 publications.

    • Park SR  et al. Sonic Hedgehog, a Novel Endogenous Damage Signal, Activates Multiple Beneficial Functions of Human Endometrial Stem Cells. Mol Ther 28:452-465 (2020). PubMed: 31866117
    • Lee NH  et al. SERPINB2 Is a Novel Indicator of Cancer Stem Cell Tumorigenicity in Multiple Cancer Types. Cancers (Basel) 11:N/A (2019). PubMed: 30965654
    • Zhang XM  et al. SERPINB2 overexpression inhibited cell proliferation, invasion and migration, led to G2/M arrest, and increased radiosensitivity in nasopharyngeal carcinoma cells. J Radiat Res 60:318-327 (2019). PubMed: 30864656
    • Cho A  et al. An Endogenous Anti-aging Factor, Sonic Hedgehog, Suppresses Endometrial Stem Cell Aging through SERPINB2. Mol Ther 27:1286-1298 (2019). PubMed: 31080015
    • Lee NH  et al. SERPINB2 is a novel indicator of stem cell toxicity. Cell Death Dis 9:724 (2018). PubMed: 29925837
    • Mertens TCJ  et al. Cigarette smoke differentially affects IL-13-induced gene expression in human airway epithelial cells. Physiol Rep 5:N/A (2017). WB . PubMed: 28701525
    • Peng H  et al. microRNA-31/factor-inhibiting hypoxia-inducible factor 1 nexus regulates keratinocyte differentiation. Proc Natl Acad Sci U S A 109:14030-4 (2012). WB, IHC-P ; Human . PubMed: 22891326
    • Angelucci S  et al. Proteome analysis of human Wharton's jelly cells during in vitro expansion. Proteome Sci 8:18 (2010). WB ; Human . PubMed: 20346146

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    Western blot abreview for Anti-SerpinB2/PAI-2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Bxpc3)
    Gel Running Conditions
    Reduced Denaturing (4-20% tris-Glycin gel)
    Loading amount
    100000 cells
    Specification
    Bxpc3
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 12 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.