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    serpinb2pai-2-antibody-epr22924-55-bsa-and-azide-free-detector-ab272770.pdf

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RecombinantRabMAb

Recombinant Anti-SerpinB2/PAI-2 antibody [EPR22924-55] - BSA and Azide free (Detector) (ab272770)

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Anti-SerpinB2/PAI-2 antibody [EPR22924-55] - BSA and Azide free (Detector) (ab272770)

    Key features and details

    • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
    • Rabbit monoclonal [EPR22924-55] to SerpinB2/PAI-2 - BSA and Azide free (Detector)
    • Suitable for: Sandwich ELISA
    • Reacts with: Human

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    Overview

    • Product name

      Anti-SerpinB2/PAI-2 antibody [EPR22924-55] - BSA and Azide free (Detector)
      See all SerpinB2/PAI-2 primary antibodies
    • Description

      Rabbit monoclonal [EPR22924-55] to SerpinB2/PAI-2 - BSA and Azide free (Detector)
    • Host species

      Rabbit
    • Tested applications

      Suitable for: Sandwich ELISAmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

    • General notes

      ab272770 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Serpin B2. The recommended pair for sandwich ELISA is:
           Capture: ab272769, Human Serpin B2 Capture Antibody (unconjugated)
           Detector: ab272770, Human Serpin B2 Detector Antibody (unconjugated)
      The reference range value is 312.5 - 20000 pg/ml.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free production
      For more information see here.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Constituent: 100% PBS
    • Carrier free

      Yes
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      EPR22924-55
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Protease inhibitors
      • Serine protease inhibitors
      • SERPINs

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
      • Goat Anti-Rabbit IgG H&L (Biotin) (ab207995)
      • Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
    • Conjugation kits

      • Alkaline phosphatase Conjugation Kit - Lightning-Link® (ab102850)
      • HRP Conjugation Kit - Lightning-Link® (ab102890)
      • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
    • sELISA pair antibody

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
      • Anti-SerpinB2/PAI-2 antibody [EPR22924-238] - BSA and Azide free (Capture) (ab272769)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab272770 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA
    Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR22924-238] to SerpinB2/PAI-2 - BSA and Azide free (Capture) (ab272769).
    Notes
    Sandwich ELISA
    Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR22924-238] to SerpinB2/PAI-2 - BSA and Azide free (Capture) (ab272769).

    Target

    • Function

      Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.
    • Sequence similarities

      Belongs to the serpin family. Ov-serpin subfamily.
    • Post-translational
      modifications

      The signal sequence is not cleaved.
    • Cellular localization

      Cytoplasm. Secreted > extracellular space.
    • Target information above from: UniProt accession P05120 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5055 Human
      • Omim: 173390 Human
      • SwissProt: P05120 Human
      • Unigene: 594481 Human
      • Alternative names

        • HsT1201 antibody
        • Monocyte Arg serpin antibody
        • Monocyte Arg-serpin antibody
        • Monocyte Arginine-serpin antibody
        • Monocyte-derived plasminogen activator inhibitor antibody
        • PAI antibody
        • PAI-2 antibody
        • PAI2 antibody
        • PAI2_HUMAN antibody
        • Placental plasminogen activator inhibitor antibody
        • PLANH2 antibody
        • Plasminogen activator inhibitor 2 antibody
        • plasminogen activator inhibitor, type 2 antibody
        • plasminogen activator inhibitor, type II (arginine-serpin) antibody
        • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2 antibody
        • Serpin B2 antibody
        • serpin peptidase inhibitor, clade B (ovalbumin), member 2 antibody
        • Serpinb2 antibody
        • Urokinase inhibitor antibody
        see all

      Images

      • Anti-SerpinB2/PAI-2 antibody [EPR22924-55] - BSA and Azide free (Detector) (ab272770)
        Anti-SerpinB2/PAI-2 antibody [EPR22924-55] - BSA and Azide free (Detector) (ab272770)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab272770? Please let us know so that we can cite the reference in this datasheet.

      ab272770 has not yet been referenced specifically in any publications.

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