Recombinant Anti-SerpinB2/PAI-2 antibody [EPR22924-56] - BSA and Azide free (ab269368)
Overview
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Product name
Anti-SerpinB2/PAI-2 antibody [EPR22924-56] - BSA and Azide free
See all SerpinB2/PAI-2 primary antibodies -
Description
Rabbit monoclonal [EPR22924-56] to SerpinB2/PAI-2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human SerpinB2/PAI-2 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P05120 -
Positive control
- WB: U-937 and A431 whole cell lysate; Human placenta lysate. IHC-P: Human skin and tonsil tissue.
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General notes
ab269368 is the carrier-free version of ab267463. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab269368 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22924-56 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab269368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
Target
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Function
Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1. -
Sequence similarities
Belongs to the serpin family. Ov-serpin subfamily. -
Post-translational
modificationsThe signal sequence is not cleaved. -
Cellular localization
Cytoplasm. Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 5055 Human
- Omim: 173390 Human
- SwissProt: P05120 Human
- Unigene: 594481 Human
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Alternative names
- HsT1201 antibody
- Monocyte Arg serpin antibody
- Monocyte Arg-serpin antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] - BSA and Azide free (ab269368)](https://www.abcam.com/ps/products/269/ab269368/Images/ab269368-2-anti-serpinb2-pai-2-antibody-epr22924-56-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] - BSA and Azide free (ab269368)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling SerpinB2/PAI-2 with ab267463 at 1/4000 dilution (0.121 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil. The section was incubated with ab267463 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267463).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] - BSA and Azide free (ab269368)](https://www.abcam.com/ps/products/269/ab269368/Images/ab269368-1-anti-serpinb2-pai-2-antibody-epr22924-56-immunohistochemistry-skin-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB2/PAI-2 antibody [EPR22924-56] - BSA and Azide free (ab269368)Immunohistochemical analysis of paraffin-embedded human skin tissue labeling SerpinB2/PAI-2 with ab267463 at 1/4000 dilution (0.121 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human skin. The section was incubated with ab267463 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with ematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267463).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References
ab269368 has not yet been referenced specifically in any publications.
