Overview

  • Product name

    Anti-SerpinB3/SCCA antibody
    See all SerpinB3/SCCA primary antibodies
  • Description

    Rabbit polyclonal to SerpinB3/SCCA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human SerpinB3/SCCA aa 89-359.
    Database link: P29508

  • Positive control

    • A549, HepG2 and HCT116 cell lysates; Human hepatoma tissue; A549 cells.
  • General notes

    Protein previously labeled as SerpinB3.

Properties

Applications

Our Abpromise guarantee covers the use of ab154971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    May act as a protease inhibitor to modulate the host immune response against tumor cells.
  • Tissue specificity

    Squamous cells. Expressed in some hepatocellular carcinoma (at protein level).
  • Sequence similarities

    Belongs to the serpin family. Ov-serpin subfamily.
  • Developmental stage

    Its expression is closely related to cellular differentiation in both normal and malignant squamous cells.
  • Cellular localization

    Cytoplasm. Seems to also be secreted in plasma by cancerous cells but at a low level.
  • Information by UniProt
  • Database links

  • Alternative names

    • HsT1196 antibody
    • Protein T4 A antibody
    • Protein T4-A antibody
    • SCC antibody
    • SCCA antibody
    • SCCA PD antibody
    • SCCA-1 antibody
    • SCCA-PD antibody
    • SCCA1 antibody
    • SCCAPD antibody
    • Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 3 antibody
    • Serpin B3 antibody
    • Serpin peptidase inhibitor clade B (ovalbumin) member 3 antibody
    • serpin peptidase inhibitor, clade B (ovalbumin), member 3 antibody
    • SERPINB3 antibody
    • SPB3_HUMAN antibody
    • Squamous cell carcinoma antigen 1 antibody
    • T4 A antibody
    • T4-A antibody
    • T4A antibody
    see all

Images

  • Anti-SerpinB3/SCCA antibody (ab154971) at 1/3000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 45 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling SerpinB3/SCCA with ab154971 at 1/500 dilution.

  • Immunofluorescent analysis of methanol-fixed A549 cells labeling SerpinB3/SCCA with ab154971 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

References

This product has been referenced in:

  • Luo D  et al. Oncogenic Ras/squamous cell carcinoma antigen signaling pathway activation promotes invasiveness and lymph node metastases in papillary thyroid carcinoma. Oncol Rep 41:1253-1263 (2019). Read more (PubMed: 30535496) »
  • An T  et al. Comparative analysis of proteomes between diabetic and normal human sperm: Insights into the effects of diabetes on male reproduction based on the regulation of mitochondria-related proteins. Mol Reprod Dev 85:7-16 (2018). Read more (PubMed: 29149484) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

I can confirm that although we have not tested these antibodies for cross-reactivity with Serpin B4 but there is high percentage of immunogen sequence homology. It means the chances of cross reactivity are high.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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