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    serpine2pn-1-antibody-ab154591.pdf

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Neuroscience Cell Adhesion Proteins Proteases
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Anti-SERPINE2/PN-1 antibody (ab154591)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

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Western blot - Anti-SERPINE2/PN-1 antibody (ab154591)
  • Western blot - Anti-SERPINE2/PN-1 antibody (ab154591)
  • Immunocytochemistry/ Immunofluorescence - Anti-SERPINE2/PN-1 antibody (ab154591)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINE2/PN-1 antibody (ab154591)

Key features and details

  • Rabbit polyclonal to SERPINE2/PN-1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Recombinant Human SERPINE2/PN-1 protein (His tag) (ab200492)

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Overview

  • Product name

    Anti-SERPINE2/PN-1 antibody
    See all SERPINE2/PN-1 primary antibodies
  • Description

    Rabbit polyclonal to SERPINE2/PN-1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human SERPINE2/PN-1 aa 163-385.
    Database link: P07093

  • Positive control

    • HeLa, HepG2 and Mouse brain whole cell lysates; HeLa cells; Human gastric carcinoma tissue.
  • General notes

    Previously labelled as SERPINE2. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Proteases
    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Mouse brain tissue lysate - total protein (ab30151)
  • Recombinant Protein

    • Recombinant Human SERPINE2/PN-1 protein (His tag) (ab200492)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Mouse
Human
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
IHC-P
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
IHC-P
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.
  • Sequence similarities

    Belongs to the serpin family.
  • Cellular localization

    Secreted > extracellular space.
  • Target information above from: UniProt accession P07093 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5270 Human
    • Entrez Gene: 20720 Mouse
    • Omim: 177010 Human
    • SwissProt: P07093 Human
    • SwissProt: Q07235 Mouse
    • Unigene: 38449 Human
    • Unigene: 708453 Human
    • Unigene: 3093 Mouse
    • Alternative names

      • GDN antibody
      • GDN_HUMAN antibody
      • GDNPF antibody
      • Glia derived nexin antibody
      • Glia-derived nexin antibody
      • Glial derived neurite promoting factor antibody
      • P17 antibody
      • Peptidase inhibitor 7 antibody
      • Pi-7 antibody
      • Plasminogen activator inhibitor type 1, member 2 antibody
      • PN-1 antibody
      • PN1 antibody
      • PNI antibody
      • Protease inhibitor 7 antibody
      • Protease nexin 1 antibody
      • Protease nexin I antibody
      • Serpin E2 antibody
      • Serpin family E member 2 antibody
      • Serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2 antibody
      • SERPINE 2 antibody
      • Serpine2 antibody
      see all

    Images

    • Western blot - Anti-SERPINE2/PN-1 antibody (ab154591)
      Western blot - Anti-SERPINE2/PN-1 antibody (ab154591)
      All lanes : Anti-SERPINE2/PN-1 antibody (ab154591) at 1/2000 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : HepG2 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 44 kDa



      10% SDS PAGE
    • Western blot - Anti-SERPINE2/PN-1 antibody (ab154591)
      Western blot - Anti-SERPINE2/PN-1 antibody (ab154591)
      Anti-SERPINE2/PN-1 antibody (ab154591) at 1/2000 dilution + Mouse brain whole cell lysate at 50 µg

      Predicted band size: 44 kDa



      10% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-SERPINE2/PN-1 antibody (ab154591)
      Immunocytochemistry/ Immunofluorescence - Anti-SERPINE2/PN-1 antibody (ab154591)

      Immunofluorescence analysis of methanol-fixed HeLa cells labeling SERPINE2/PN-1 with ab154591 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINE2/PN-1 antibody (ab154591)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINE2/PN-1 antibody (ab154591)

      Immunohistochemical analysis of paraffin-embedded Human Gastric carcinoma tissue labeling SERPINE2/PN-1 with ab154591 at 1/500 dilution.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab154591? Please let us know so that we can cite the reference in this datasheet.

    ab154591 has been referenced in 2 publications.

    • Li YB  et al. miR-199a-3p is involved in the pathogenesis and progression of diabetic neuropathy through downregulation of SerpinE2. Mol Med Rep 16:2417-2424 (2017). PubMed: 28677735
    • Wu X  et al. The Involvement of Protease Nexin-1 (PN1) in the Pathogenesis of Intervertebral Disc (IVD) Degeneration. Sci Rep 6:30563 (2016). PubMed: 27460424

    Customer reviews and Q&As

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