Anti-SERPINE2/PN-1 antibody (ab154591)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-SERPINE2/PN-1 antibody
See all SERPINE2/PN-1 primary antibodies -
Description
Rabbit polyclonal to SERPINE2/PN-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human SERPINE2/PN-1 aa 163-385.
Database link: P07093 -
Positive control
- HeLa, HepG2 and Mouse brain whole cell lysates; HeLa cells; Human gastric carcinoma tissue.
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General notes
Previously labelled as SERPINE2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 44 kDa. | |
| IHC-P | 1/100 - 1/1000. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin. -
Sequence similarities
Belongs to the serpin family. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 5270 Human
- Entrez Gene: 20720 Mouse
- Omim: 177010 Human
- SwissProt: P07093 Human
- SwissProt: Q07235 Mouse
- Unigene: 38449 Human
- Unigene: 708453 Human
- Unigene: 3093 Mouse
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Alternative names
- GDN antibody
- GDN_HUMAN antibody
- GDNPF antibody
see all
Images
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Western blot - Anti-SERPINE2/PN-1 antibody (ab154591)All lanes : Anti-SERPINE2/PN-1 antibody (ab154591) at 1/2000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 44 kDa
10% SDS PAGE -
Western blot - Anti-SERPINE2/PN-1 antibody (ab154591)Anti-SERPINE2/PN-1 antibody (ab154591) at 1/2000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 44 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-SERPINE2/PN-1 antibody (ab154591)Immunofluorescence analysis of methanol-fixed HeLa cells labeling SERPINE2/PN-1 with ab154591 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINE2/PN-1 antibody (ab154591)Immunohistochemical analysis of paraffin-embedded Human Gastric carcinoma tissue labeling SERPINE2/PN-1 with ab154591 at 1/500 dilution.
Protocols
References
This product has been referenced in:
- Li YB et al. miR-199a-3p is involved in the pathogenesis and progression of diabetic neuropathy through downregulation of SerpinE2. Mol Med Rep 16:2417-2424 (2017). Read more (PubMed: 28677735) »
- Wu X et al. The Involvement of Protease Nexin-1 (PN1) in the Pathogenesis of Intervertebral Disc (IVD) Degeneration. Sci Rep 6:30563 (2016). Read more (PubMed: 27460424) »
