Anti-SERPINE2/PN-1 antibody (ab154591)
Key features and details
- Rabbit polyclonal to SERPINE2/PN-1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SERPINE2/PN-1 antibody
See all SERPINE2/PN-1 primary antibodies -
Description
Rabbit polyclonal to SERPINE2/PN-1 -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P HumanWB MouseHuman -
Immunogen
Recombinant fragment corresponding to Human SERPINE2/PN-1 aa 163-385.
Database link: P07093 -
Positive control
- HeLa, HepG2 and Mouse brain whole cell lysates; HeLa cells; Human gastric carcinoma tissue.
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General notes
Previously labelled as SERPINE2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC/IF |
Human
|
IHC-P |
Human
|
WB |
Mouse
Human
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
|
|
IHC-P |
1/100 - 1/1000.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 44 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin. -
Sequence similarities
Belongs to the serpin family. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 5270 Human
- Entrez Gene: 20720 Mouse
- Omim: 177010 Human
- SwissProt: P07093 Human
- SwissProt: Q07235 Mouse
- Unigene: 38449 Human
- Unigene: 708453 Human
- Unigene: 3093 Mouse
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Alternative names
- GDN antibody
- GDN_HUMAN antibody
- GDNPF antibody
see all
Images
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All lanes : Anti-SERPINE2/PN-1 antibody (ab154591) at 1/2000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 44 kDa
10% SDS PAGE -
Anti-SERPINE2/PN-1 antibody (ab154591) at 1/2000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 44 kDa
10% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells labeling SERPINE2/PN-1 with ab154591 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINE2/PN-1 antibody (ab154591)
Immunohistochemical analysis of paraffin-embedded Human Gastric carcinoma tissue labeling SERPINE2/PN-1 with ab154591 at 1/500 dilution.
Protocols
References (2)
ab154591 has been referenced in 2 publications.
- Li YB et al. miR-199a-3p is involved in the pathogenesis and progression of diabetic neuropathy through downregulation of SerpinE2. Mol Med Rep 16:2417-2424 (2017). PubMed: 28677735
- Wu X et al. The Involvement of Protease Nexin-1 (PN1) in the Pathogenesis of Intervertebral Disc (IVD) Degeneration. Sci Rep 6:30563 (2016). PubMed: 27460424