Anti-SERPINE2/PN-1 antibody (ab154591)

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Western blot - Anti-SERPINE2/PN-1 antibody (ab154591)
  • Western blot - Anti-SERPINE2/PN-1 antibody (ab154591)
  • Immunocytochemistry/ Immunofluorescence - Anti-SERPINE2/PN-1 antibody (ab154591)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINE2/PN-1 antibody (ab154591)

Key features and details

  • Rabbit polyclonal to SERPINE2/PN-1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-SERPINE2/PN-1 antibody
    See all SERPINE2/PN-1 primary antibodies

  • Description

    Rabbit polyclonal to SERPINE2/PN-1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human SERPINE2/PN-1 aa 163-385.
    Database link: P07093

  • Positive control

    • HeLa, HepG2 and Mouse brain whole cell lysates; HeLa cells; Human gastric carcinoma tissue.

  • General notes

    Previously labelled as SERPINE2. 

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Mouse
Human
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
IHC-P
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
IHC-P
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.

  • Sequence similarities

    Belongs to the serpin family.

  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

  • Database links

    • Alternative names

      • GDN antibody
      • GDN_HUMAN antibody
      • GDNPF antibody
      • Glia derived nexin antibody
      • Glia-derived nexin antibody
      • Glial derived neurite promoting factor antibody
      • P17 antibody
      • Peptidase inhibitor 7 antibody
      • Pi-7 antibody
      • Plasminogen activator inhibitor type 1, member 2 antibody
      • PN-1 antibody
      • PN1 antibody
      • PNI antibody
      • Protease inhibitor 7 antibody
      • Protease nexin 1 antibody
      • Protease nexin I antibody
      • Serpin E2 antibody
      • Serpin family E member 2 antibody
      • Serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2 antibody
      • SERPINE 2 antibody
      • Serpine2 antibody
      see all

    Images

    • Western blot - Anti-SERPINE2/PN-1 antibody (ab154591)
      Western blot - Anti-SERPINE2/PN-1 antibody (ab154591)
      All lanes : Anti-SERPINE2/PN-1 antibody (ab154591) at 1/2000 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : HepG2 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 44 kDa



      10% SDS PAGE
    • Western blot - Anti-SERPINE2/PN-1 antibody (ab154591)
      Western blot - Anti-SERPINE2/PN-1 antibody (ab154591)
      Anti-SERPINE2/PN-1 antibody (ab154591) at 1/2000 dilution + Mouse brain whole cell lysate at 50 µg

      Predicted band size: 44 kDa



      10% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-SERPINE2/PN-1 antibody (ab154591)
      Immunocytochemistry/ Immunofluorescence - Anti-SERPINE2/PN-1 antibody (ab154591)

      Immunofluorescence analysis of methanol-fixed HeLa cells labeling SERPINE2/PN-1 with ab154591 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINE2/PN-1 antibody (ab154591)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINE2/PN-1 antibody (ab154591)

      Immunohistochemical analysis of paraffin-embedded Human Gastric carcinoma tissue labeling SERPINE2/PN-1 with ab154591 at 1/500 dilution.

    References (2)

    Publishing research using ab154591? Please let us know so that we can cite the reference in this datasheet.

    ab154591 has been referenced in 2 publications.

    • Li YB  et al. miR-199a-3p is involved in the pathogenesis and progression of diabetic neuropathy through downregulation of SerpinE2. Mol Med Rep 16:2417-2424 (2017). PubMed: 28677735
    • Wu X  et al. The Involvement of Protease Nexin-1 (PN1) in the Pathogenesis of Intervertebral Disc (IVD) Degeneration. Sci Rep 6:30563 (2016). PubMed: 27460424

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