Overview

  • Product name
    Anti-Serum Amyloid A antibody [115]
    See all Serum Amyloid A primary antibodies
  • Description
    Mouse monoclonal [115] to Serum Amyloid A
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly purified recombinant human serum amyloid A (MW: 12 kDa).

  • General notes

    This product was changed from ascites to tissue culture supernatant on 6th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Use at an assay dependent dilution. This antibody, at a concentration of 0.1-0.5 µg/ml, will allow visualisation of 100 ng/lane of recombinant human SAA.
Sandwich ELISA Use at an assay dependent concentration.

sELISA compatible pair

Target

  • Relevance
    Function: Major acute phase reactant. Apolipoprotein of the HDL complex. Tissue specificity: Expressed by the liver; secreted in plasma. Disease: Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Note=Elevated serum SAA protein levels may be associated with lung cancer. Similarity: Belongs to the SAA family. PTM: This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.
  • Database links
  • Alternative names
    • Amyloid fibrils antibody
    • SAA 2 antibody
    • Serum amyloid A1 isoform 2 antibody
    • TP53I4 antibody
    • Amyloid fibril protein AA antibody
    • Amyloid protein A antibody
    • Fibrils antibody
    • MGC111216 antibody
    • OC antibody
    • PIG 4 antibody
    • PIG4 antibody
    • SAA 1 antibody
    • SAA antibody
    • SAA_HUMAN antibody
    • SAA1 antibody
    • SAA2 antibody
    • Serum amyloid A protein precursor antibody
    • Serum amyloid A1 isoform 1 antibody
    • Serum amyloid protein A(4-101) antibody
    • Tumor protein p53 inducible protein 4 antibody
    see all

Images

  • All lanes : Anti-Serum Amyloid A antibody [115] (ab687) at 1/4000 dilution

    Lanes 1-4 : Acute phase plasma samples
    Lane 5 : Recombinant C-SAA
    Lane 6 : Recombinant A-SAA

    Lysates/proteins at 14 µg per lane.

    Secondary
    All lanes : Goat anti-mouse IgG-HRP at 1/10000 dilution

    Developed using the ECL technique.

    Exposure time: 5 seconds

References

This product has been referenced in:
  • Heo SH  et al. Comparative proteomic analysis in children with idiopathic short stature (ISS) before and after short-term recombinant human growth hormone (rhGH) therapy. Proteomics 13:1211-9 (2013). Human . Read more (PubMed: 23436750) »
  • Jahangiri A  et al. Serum amyloid A is found on ApoB-containing lipoproteins in obese humans with diabetes. Obesity (Silver Spring) 21:993-6 (2013). Read more (PubMed: 23784902) »
See all 5 Publications for this product

Customer reviews and Q&As

1-10 of 11 Q&A

Answer

Thank you for contacting us.

I have now received further information from developer lab regarding the products ab687 and ab18711. Regrettably we will not be able to recommend these antibodies, to be used with horse samples. I apologies for any inconvenience.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your email.

Finally, I have checked the sequence homology between human and horse SAA proteins, the homology is quite low with matching stretches.

http://www.uniprot.org/blast/uniprot/2012101621EV411P6G?alignment=27


P0DJI9 is for human SSA protein and F6PJF6 is the ID for horse protein.

In order to further check whether the antibodies ab687 and ab18711 would be suitable, I have contacted the developer laboratory of these antibodies and asked if they can match the epitope of these antibodies with horse protein sequence. I will send you the details soon.

The other antibodies we have in catalogue are mostly against the full length human proteins so I am sorry we may not be able to support the horse species cross reactivity. We however have rabbit monoclonal that have >95% immunogen sequence homology with horse so this antibody should cross react with horse.

https://www.abcam.com/Serum-Amyloid-A-antibody-EPR4134-ab108992.html


Along with ab108992 you can search an anti SAA antibody which detects N-terminal end of protein, to use these antibodies in sELISA. I am sorry we do not have N-terminal specific products.


I hope this information will nevertheless helpful. Please do not hesitate to contact me with further inquiries.

Read More

Answer

Thank you for contacting us.

We can recommend paring between ab687 and ab18711 instead of ab18713

https://www.abcam.com/serum-Amyloid-A-antibody-607-ab18711.html

https://www.abcam.com/Serum-Amyloid-A-antibody-115-ab687.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us. The antibody ab687 is reactive towards the Serum Amyloid A protein and not specific variants.. We do not have any antibodies specific for SAA1,SAA2 or SAA4.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.

I have looked into what applications anti-Serum Amyloid A antibodies ab687 and ab81483 have been used for in order to ascertain if they are likely to detect the native epitope of Serum Amyloid A in order to perform native western blotting.

Both ab687 and ab81483 have been used in ELISA where the native epitope is presented. The ELISA carried out with ab687 was performed with the endogenously expressed protein, whilst the ELISA performed with ab81483 used recombinantly expressed protein. Both of these proteins are therefore likely to also be able to detect the protein when performing a native western blot. However, as we have not carried out this experiment I cannot guarantee that it will.

I also looked into what protocol had been used in performing immunoprecipitation with ab81483, however, the lab were unfortunately able to confirm that they had not tested the antibody in this application and that the informationhad been added to the datasheet in error. The datasheet has now been updated to reflect this.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Answer

Thank you for your phone call. 1) Regarding the image for ab1793, this was submitted by a customer as Abreview. For the details of the Abreview please follow this link: https://www.abcam.com/index.html?datasheet=1793&tab=abreviews&intabreviewid=17818 It might well be that the monoclonal rabbit IgG secondary antibody is part of the DAKO EnVision+ System, Peroxidase/DAB kit mentioned in the Abreview. But unfortunately, I do not have more information in this regard. ------------------------------------------------------------------------- 2) As for the testing discount for ab687, here is the necessary information: DISCOUNT CODE: xxx Expiration date: xxx I am very pleased to hear you would like to accept our offer and test ab687 in mouse. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. I hope this information is of help to you. Please let me know if I can assist you further with our products.

Read More

Question
Answer

Thank you for your enquiry. Unfortunately, ab687 has not been tested for reactivity with SAA4. If you have any additional questions, please contact us again.

Read More

Answer

Thank you very much for your patience regarding this issue. I wanted to let you know that a colleague and myself are investigating this problem with the originator of this antibody. If a product is not functioning as cited on our product data sheet the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience. In the meantime, I would like to offer you a refund or credit note for your original order for ab687 or I can send you an alternative antibody of your choice. Please let me know which you would prefer, and I look forward to hearing from you.

Read More

Question
Answer

Thank you for your enquiry. Ab687 can be used as the capture antibody in a sandwich ELISA. Clone 607 Serum Amyloid A antibodies can be used as the detection antibodies. However, we do not currently carry this clone. If you have any more questions, please contact us again.

Read More

Answer

Unfortunately, because we use the entire, intact protein antigen to raise the antibodies, we have no way of knowing whether the antibody is directed at the SAA carboxy terminus.

Read More

1-10 of 11 Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up