Overview

  • Product name
    Anti-Serum Amyloid A antibody [EP11592-92]
    See all Serum Amyloid A primary antibodies
  • Description
    Rabbit monoclonal [EP11592-92] to Serum Amyloid A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Serum Amyloid A aa 1-100. The exact sequence is proprietary.
    Database link: P0DJI8

  • Positive control
    • Human fetal liver lysate. Human serum and plasma lysates. Human hepatocellular carcinoma and Human liver tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab190802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance
    Function: Major acute phase reactant. Apolipoprotein of the HDL complex. Tissue specificity: Expressed by the liver; secreted in plasma. Disease: Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Note=Elevated serum SAA protein levels may be associated with lung cancer. Similarity: Belongs to the SAA family. PTM: This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.
  • Database links
  • Alternative names
    • Amyloid fibrils antibody
    • SAA 2 antibody
    • Serum amyloid A1 isoform 2 antibody
    • TP53I4 antibody
    • Amyloid fibril protein AA antibody
    • Amyloid protein A antibody
    • Fibrils antibody
    • MGC111216 antibody
    • OC antibody
    • PIG 4 antibody
    • PIG4 antibody
    • SAA 1 antibody
    • SAA antibody
    • SAA_HUMAN antibody
    • SAA1 antibody
    • SAA2 antibody
    • Serum amyloid A protein precursor antibody
    • Serum amyloid A1 isoform 1 antibody
    • Serum amyloid protein A(4-101) antibody
    • Tumor protein p53 inducible protein 4 antibody
    see all

Images

  • All lanes : Anti-Serum Amyloid A antibody [EP11592-92] (ab190802) at 1/2000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human plasma lysate
    Lane 3 : Human serum lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 14 kDa
    Observed band size: 12 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue sections labeling Serum Amyloid A using ab190802 at a 1/1000 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

  • Immunohistochemical analysis of paraffin embedded Human liver tissue sections labeling Serum Amyloid A using ab190802 at a 1/1000 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

References

This product has been referenced in:
  • Lv P  et al. Apolipoprotein C-III in the high-density lipoprotein proteome of cerebral lacunar infarction patients impairs its anti-inflammatory function. Int J Mol Med 41:61-68 (2018). Read more (PubMed: 29115584) »
See 1 Publication for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse breast cancer cell line, Mouse breast tumour)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
50 µg
Specification
Mouse breast cancer cell line, Mouse breast tumour
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Nov 21 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up