Anti-Serum Amyloid A antibody [EP11592-92] - BSA and Azide free (ab235995)

Overview

  • Product name

    Anti-Serum Amyloid A antibody [EP11592-92] - BSA and Azide free
    See all Serum Amyloid A primary antibodies
  • Description

    Rabbit monoclonal [EP11592-92] to Serum Amyloid A - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Serum Amyloid A aa 1-100. The exact sequence is proprietary.
    Database link: P0DJI8

  • Positive control

    • IHC-P: Human liver tissue.
  • General notes

    Ab235995 is the carrier-free version of ab190802. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab235995 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab235995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 12 kDa (predicted molecular weight: 14 kDa).

Target

  • Relevance

    Function: Major acute phase reactant. Apolipoprotein of the HDL complex. Tissue specificity: Expressed by the liver; secreted in plasma. Disease: Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Note=Elevated serum SAA protein levels may be associated with lung cancer. Similarity: Belongs to the SAA family. PTM: This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.
  • Database links

  • Alternative names

    • Amyloid fibrils antibody
    • SAA 2 antibody
    • Serum amyloid A1 isoform 2 antibody
    • TP53I4 antibody
    • Amyloid fibril protein AA antibody
    • Amyloid protein A antibody
    • Fibrils antibody
    • MGC111216 antibody
    • OC antibody
    • OTTMUSP0000004557 antibody
    • PIG 4 antibody
    • PIG4 antibody
    • SAA 1 antibody
    • SAA antibody
    • SAA_HUMAN antibody
    • SAA1 antibody
    • SAA2 antibody
    • Serum amyloid A 1 antibody
    • serum amyloid A antibody
    • Serum amyloid A protein precursor antibody
    • Serum amyloid A1 isoform 1 antibody
    • Serum amyloid protein A(4-101) antibody
    • Tumor protein p53 inducible protein 4 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue sections labeling Serum Amyloid A using ab190802 at a 1/1000 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190802).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin embedded Human liver tissue sections labeling Serum Amyloid A using ab190802 at a 1/1000 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190802).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

References

ab235995 has not yet been referenced specifically in any publications.

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