Overview

  • Product name
    Anti-Serum Amyloid P / SAP antibody [14B4]
    See all Serum Amyloid P / SAP primary antibodies
  • Description
    Mouse monoclonal [14B4] to Serum Amyloid P / SAP
  • Host species
    Mouse
  • Specificity
    None detected so far.
  • Tested applications
    Suitable for: Sandwich ELISA, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Serum Amyloid P/ SAP.

  • General notes

     

    This antibody reacts with Serum Amyloid P / SAP coated on microtitre plates. In western blots of SDS-PAGE separated serum proteins, the antibody reacts with a high molecular weight aggregate of Serum Amyloid P / SAP under non reducing conditions, and reacts strongly with a 25 kDa band of the single subunits under reducing SDS-PAGE conditions.

    Previously labelled as Serum Amyloid P.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 0.0268% PBS, 2.9% Sodium chloride
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    This antibody reacts with serum Amyloid P coated on microtitre plates. In western blots of SDS-PAGE separated serum proteins, the antibody reacts with a high molecular weight aggregate of serum Amyloid P under non reducing conditions, and reacts strongly with a 25 kDa band of the single subunits under reducing SDS-PAGE conditions.
  • Clonality
    Monoclonal
  • Clone number
    14B4
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA 1/4000. Can be paired for Sandwich ELISA with Rabbit polyclonal to Serum Amyloid P / SAP (ab17872). Use as Capture antibody with recommended pair. See Abreview submitted by Jennifer Webster for details.
WB 1/400. Use under non reducing condition. Predicted molecular weight: 25 kDa.
ELISA 1/4000. Use at an assay dependent dilution.

Target

  • Function
    Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
  • Tissue specificity
    Found in serum and urine.
  • Involvement in disease
    Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.
  • Sequence similarities
    Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • Post-translational
    modifications
    N-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148).
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9.5S alpha 1 glycoprotein antibody
    • 9.5S alpha-1-glycoprotein antibody
    • Amyloid P component serum antibody
    • APCS antibody
    • MGC88159 antibody
    • Pentaxin related antibody
    • Pentraxin 2 antibody
    • PTX 2 antibody
    • PTX2 antibody
    • SAMP_HUMAN antibody
    • SAP antibody
    • Serum Amyloid P antibody
    • Serum amyloid P component antibody
    • Serum amyloid P-component(1-203) antibody
    see all

References

This product has been referenced in:
  • He X  et al. An Improved Method for the Sensitive Detection of Shiga Toxin 2 in Human Serum. Toxins (Basel) 10:N/A (2018). Read more (PubMed: 29385045) »
  • Lambert C  et al. Phytosterols and Omega 3 Supplementation Exert Novel Regulatory Effects on Metabolic and Inflammatory Pathways: A Proteomic Study. Nutrients 9:N/A (2017). WB ; Human . Read more (PubMed: 28608804) »
See all 4 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for providing me these additional details.

I am sorry to confirm that we don't have any different lots in stock at the moment. I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch. Regrettably, I can suggest you have received a bad vial on this occasion.
Your order was for four vials of this product, were you able to test any of the additional vials to see is they reacted in a similar way?

I hope this will be helpful. I look forward to hearing from you with details of how you would like to proceed.

Read More

Answer

Thank you for contacting us.

I am sorry to hear you have been experiencing problems with one of our antibodies. The quality of our products is important to us and I would like to reassure you that we investigate all customer complaints. I appreciate the time taken to submit further information to us, and after reading the answers provided I would like to ask some additional questions. We currently do not have any other lot of this product.

To determine whether this product is covered by our Abpromise guarantee I would appreciate it if you could provide me information about your experiments including the tissue type, application and specifics about the protocol that you use.

I look forward to receiving your reply. Therefore if suggestions do not improve the results I would be happy to offer a refund or replacement vials, please keep the unopened vials at -20C as we may ask to have them returned to us. Please do not hesitate to contact me should you have additional questions.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (Purified protein)
Loading amount
0.1 µg
Specification
Purified protein
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Mr. John Andersen

Verified customer

Submitted Mar 01 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Serum (Serum)
Specification
Serum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Type
Sandwich

Miss. Jennifer Webster

Verified customer

Submitted Aug 16 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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