Anti-Serum Amyloid P/SAP antibody (ab233265)
Key features and details
- Rabbit polyclonal to Serum Amyloid P/SAP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Serum Amyloid P/SAP antibody
See all Serum Amyloid P/SAP primary antibodies -
Description
Rabbit polyclonal to Serum Amyloid P/SAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Serum Amyloid P/SAP aa 1 to the C-terminus. N-terminal His-tag and GST-tag; Expressed in E.coli.
Database link: P02743 -
Positive control
- WB: Human serum; Human urine; Recombinant human Serum Amyloid P/SAP protein. IHC-P: Human liver, glioma, kidney, stomach cancer, liver cancer and lung cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 25 kDa.
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IHC-P |
Use a concentration of 0.5 - 2 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 25 kDa. |
IHC-P
Use a concentration of 0.5 - 2 µg/ml. |
Target
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Function
Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin. -
Tissue specificity
Found in serum and urine. -
Involvement in disease
Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits. -
Sequence similarities
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
Post-translational
modificationsN-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148). -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 325 Human
- Omim: 104770 Human
- SwissProt: P02743 Human
- Unigene: 507080 Human
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Alternative names
- 9.5S alpha 1 glycoprotein antibody
- 9.5S alpha-1-glycoprotein antibody
- Amyloid P component serum antibody
see all
Images
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Formalin-fixed, paraffin-embedded human lung cancer tissue stained for Serum Amyloid P/SAP using ab233265 at 30 ug/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human liver cancer tissue stained for Serum Amyloid P/SAP using ab233265 at 30 ug/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for Serum Amyloid P/SAP using ab233265 at 30 ug/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for Serum Amyloid P/SAP using ab233265 at 30 ug/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human glioma tissue stained for Serum Amyloid P/SAP using ab233265 at 30 ug/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human liver tissue stained for Serum Amyloid P/SAP using ab233265 at 30 ug/ml in immunohistochemical analysis. DAB staining.
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All lanes : Anti-Serum Amyloid P/SAP antibody (ab233265) at 2 µg/ml
Lane 1 : Human serum
Lane 2 : Human urine
Secondary
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa -
Anti-Serum Amyloid P/SAP antibody (ab233265) at 2 µg/ml + Recombinant human Serum Amyloid P/SAP protein
Predicted band size: 25 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233265 has not yet been referenced specifically in any publications.