Key features and details
- Mouse monoclonal [342CT9.4.7] to Serum Amyloid P/SAP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-Serum Amyloid P/SAP antibody [342CT9.4.7]
See all Serum Amyloid P/SAP primary antibodies
DescriptionMouse monoclonal [342CT9.4.7] to Serum Amyloid P/SAP
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Recombinant full length protein corresponding to Human Serum Amyloid P/SAP aa 1 to the C-terminus.
- This antibody gave a positive signal in the following human tissue lysates: plasma; spleen; bladder.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab87914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). Block using 3% milk.|
FunctionCan interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
Tissue specificityFound in serum and urine.
Involvement in diseaseNote=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.
Sequence similaritiesBelongs to the pentaxin family.
Contains 1 pentaxin domain.
modificationsN-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148).
- Information by UniProt
- 9.5S alpha 1 glycoprotein antibody
- 9.5S alpha-1-glycoprotein antibody
- Amyloid P component serum antibody
All lanes : Anti-Serum Amyloid P/SAP antibody [342CT9.4.7] (ab87914) at 5 µg/ml
Lane 1 : Human Plasma Total Protein Lysate
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : Human bladder tissue lysate - soluble fraction (female, 45 years) (ab44654)
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 8 minutes
Blocked using 3% milk.
ab87914 has been referenced in 1 publication.
- Cantres-Rosario YM et al. HIV Infection Induces Extracellular Cathepsin B Uptake and Damage to Neurons. Sci Rep 9:8006 (2019). PubMed: 31142756