Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1018Y] to Serum Amyloid P/SAP - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Product nameAnti-Serum Amyloid P/SAP antibody [EP1018Y] - BSA and Azide free
See all Serum Amyloid P/SAP primary antibodies
DescriptionRabbit monoclonal [EP1018Y] to Serum Amyloid P/SAP - BSA and Azide free
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Serum Amyloid P/SAP aa 1 to the C-terminus (N terminal). The exact sequence is proprietary.
Database link: P02743
- IHC-P: Human endometrial carcinoma tissue.
ab232468 is the carrier-free version of ab45151 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab232468 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Previously labelled as Serum Amyloid P.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab232468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Enzymatic antigen retrieval (using protease) also has been found to work and may acheive better results.
|WB||Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).|
FunctionCan interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
Tissue specificityFound in serum and urine.
Involvement in diseaseNote=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.
Sequence similaritiesBelongs to the pentaxin family.
Contains 1 pentaxin domain.
modificationsN-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148).
- Information by UniProt
- 9.5S alpha 1 glycoprotein antibody
- 9.5S alpha-1-glycoprotein antibody
- Amyloid P component serum antibody
ab45151 staining Serum Amyloid P/SAP in human endometrial carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Negative control 1: PBS in place of primary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45151).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232468 has not yet been referenced specifically in any publications.