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I am interested in purifying antibody from hamster serum. I have approximately 3-4 ml of serum. I would like to use the serum antibody purification kit (protein G) - ab128751.
What size do you recommend? 1 test or 3 test? Do I need to purchase any additional supplies or reagents?
Thank you very much for your help.
Asked on Feb 01 2013
Thank you for your enquiry. Here are the answers to the questions posed above:
question1): Does this kit (serum antibody purification kit (protein G)) - ab128751 work in hamster serum?
Yes. Protein G has moderate affinity for hamster IgG and should give results.
question 2:) If this works on hamster serum, how many kits would be necessary to purify 3-4 ml of hamster serum?
Normal hamster serum IgG levels are between 8 and 17mg/ml. This kit can bind up to 10mg of rabbit IgG therefore two to seven kits would be necessary.
question 3:) Are there any additional supplies or reagents necessary to run this kit, or with hamster?
Please let us know if you have further questions.
Answered on Feb 01 2013