Question (71511) | Serum Antibody Purification Kit (Protein G) (ab128751)

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Question

I am interested in purifying antibody from hamster serum. I have approximately 3-4 ml of serum. I would like to use the serum antibody purification kit (protein G) - ab128751.

What size do you recommend? 1 test or 3 test? Do I need to purchase any additional supplies or reagents?

Thank you very much for your help.

Answer

Thank you for your enquiry. Here are the answers to the questions posed above:


question1): Does this kit (serum antibody purification kit (protein G)) - ab128751 work in hamster serum?
Yes. Protein G has moderate affinity for hamster IgG and should give results.

question 2:) If this works on hamster serum, how many kits would be necessary to purify 3-4 ml of hamster serum?
Normal hamster serum IgG levels are between 8 and 17mg/ml. This kit can bind up to 10mg of rabbit IgG therefore two to seven kits would be necessary.
question 3:) Are there any additional supplies or reagents necessary to run this kit, or with hamster?
No.

Please let us know if you have further questions.

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