Overview

  • Product name

    Anti-Serum Response Factor SRF antibody
    See all Serum Response Factor SRF primary antibodies
  • Description

    Rabbit polyclonal to Serum Response Factor SRF
  • Host species

    Rabbit
  • Specificity

    ab53147 detects endogenous levels of total Serum Response Factor SRF protein.
  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non phosphopeptide derived from human Serum Response Factor SRF around the phosphorylation site of serine 99 (S-L-S-E-M).

  • Positive control

    • WB: NIH/3T3 cell extracts IHC-P: Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab53147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 52 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

  • Function

    SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
  • Sequence similarities

    Contains 1 MADS-box domain.
  • Post-translational
    modifications

    Phosphorylated by PRKDC.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • c fos serum response element binding factor antibody
    • c fos serum response element binding transcription factor antibody
    • ELK3 antibody
    • ERP antibody
    • MCM 1 antibody
    • MCM1 antibody
    • OTTHUMP00000039820 antibody
    • SAP2 antibody
    • Serum response factor antibody
    • SRF antibody
    • SRF serum response factor c fos serum response element binding transcription factor antibody
    • SRF_HUMAN antibody
    see all

Images

  • All lanes : Anti-Serum Response Factor SRF antibody (ab53147) at 1/300 dilution

    Lane 1 : Extracts of NIH/3T3 cells treated with PMA (125ng/ml, 30 min) with no peptide
    Lane 2 : Extracts of NIH/3T3 cells treated with PMA (125ng/ml, 30 min) with peptide

    Predicted band size: 52 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of Serum Response Factor SRF in paraffin embedded human breast carcinoma tissue using ab53147 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.

References

This product has been referenced in:

  • Hou J  et al. Role of integrin-linked kinase in the hypoxia-induced phenotypic transition of pulmonary artery smooth muscle cells: Implications for hypoxic pulmonary hypertension. Exp Cell Res 382:111476 (2019). Read more (PubMed: 31255599) »
  • Qiao J  et al. SRF promotes gastric cancer metastasis through stromal fibroblasts in an SDF1-CXCR4-dependent manner. Oncotarget 7:46088-46099 (2016). WB, IHC-P . Read more (PubMed: 27323859) »
See all 7 Publications for this product

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