Key features and details
- Rabbit polyclonal to Serum Response Factor SRF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Serum Response Factor SRF antibody
See all Serum Response Factor SRF primary antibodies
DescriptionRabbit polyclonal to Serum Response Factor SRF
SpecificityThis antibody detects endogenous levels of total Serum Response Factor SRF protein.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthesized non phosphopeptide derived from human Serum Response Factor SRF around the phosphorylation site of threonine 159 (R-Y-TP-T-F)
- Extracts from HeLa cells.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab79378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 52 kDa.|
FunctionSRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
Sequence similaritiesContains 1 MADS-box domain.
modificationsPhosphorylated by PRKDC.
- Information by UniProt
- c fos serum response element binding factor antibody
- c fos serum response element binding transcription factor antibody
- ELK3 antibody
All lanes : Anti-Serum Response Factor SRF antibody (ab79378) at 1/500 dilution
Lane 1 : Extracts from HeLa cells
Lane 2 : Extracts from HeLa cells with immunizing peptide at 10 µg
Lysates/proteins at 10 µg per lane.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
ab79378 has not yet been referenced specifically in any publications.