Overview

  • Product name

    Anti-Serum Response Factor SRF antibody
    See all Serum Response Factor SRF primary antibodies
  • Description

    Rabbit polyclonal to Serum Response Factor SRF
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous levels of total Serum Response Factor SRF protein.
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized non phosphopeptide derived from human Serum Response Factor SRF around the phosphorylation site of threonine 159 (R-Y-TP-T-F)

  • Positive control

    • Extracts from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab79378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Predicted molecular weight: 52 kDa.

Target

  • Function

    SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
  • Sequence similarities

    Contains 1 MADS-box domain.
  • Post-translational
    modifications

    Phosphorylated by PRKDC.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • c fos serum response element binding factor antibody
    • c fos serum response element binding transcription factor antibody
    • ELK3 antibody
    • ERP antibody
    • MCM 1 antibody
    • MCM1 antibody
    • OTTHUMP00000039820 antibody
    • SAP2 antibody
    • Serum response factor antibody
    • SRF antibody
    • SRF serum response factor c fos serum response element binding transcription factor antibody
    • SRF_HUMAN antibody
    see all

Images

  • All lanes : Anti-Serum Response Factor SRF antibody (ab79378) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells
    Lane 2 : Extracts from HeLa cells with immunizing peptide at 10 µg

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa
    Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.

References

ab79378 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Medaka fish Tissue lysate - other (muscle)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
10 µg
Specification
muscle
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

ゆり子 松崎

Verified customer

Submitted Sep 04 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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