Overview

  • Product name
    Anti-Serum Response Factor SRF (phospho S103) antibody
    See all Serum Response Factor SRF primary antibodies
  • Description
    Rabbit polyclonal to Serum Response Factor SRF (phospho S103)
  • Host species
    Rabbit
  • Specificity
    ab53130 detects endogenous levels of Serum Response Factor SRF only when phosphorylated at serine 103. In mouse and rat the modification site is serine 99.
  • Tested applications
    Suitable for: WB, IHC-P, ELISA, EMSAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic phosphopeptide derived from human Serum Response Factor SRF around the phosphorylation site of serine 103 (S-L-SP-E-M).

  • Positive control
    • WB: LOVO cell extracts IHC-P: Human lung carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab53130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
EMSA Use at an assay dependent concentration.

Target

  • Function
    SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
  • Sequence similarities
    Contains 1 MADS-box domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • c fos serum response element binding factor antibody
    • c fos serum response element binding transcription factor antibody
    • ELK3 antibody
    • ERP antibody
    • MCM 1 antibody
    • MCM1 antibody
    • OTTHUMP00000039820 antibody
    • SAP2 antibody
    • Serum response factor antibody
    • SRF antibody
    • SRF serum response factor c fos serum response element binding transcription factor antibody
    • SRF_HUMAN antibody
    see all

Images

  • All lanes : Anti-Serum Response Factor SRF (phospho S103) antibody (ab53130) at 1/300 dilution

    Lane 1 : LOVO cell extract treated with serum (20%, 15mins)
    Lane 2 : LOVO cell extract treated with serum (20%, 15mins) with immunising peptide

    Predicted band size: 52 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

  • ab53130 at 1/50 dilution staining Serum Response Factor SRF in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

References

This product has been referenced in:
  • Russell AP  et al. Striated muscle activator of Rho signalling (STARS) is reduced in ageing human skeletal muscle and targeted by miR-628-5p. Acta Physiol (Oxf) 220:263-274 (2017). Read more (PubMed: 27739650) »
  • Heller EA  et al. Locus-specific epigenetic remodeling controls addiction- and depression-related behaviors. Nat Neurosci 17:1720-7 (2014). Read more (PubMed: 25347353) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us. Unfortunately, this specific lysate (LOVO cell extract treated with serum (20%, 15min)) is not available for sale. Alternative positive controls would be a human lung carcinoma lysate (e.g. ab14870 or ab14871). Serum response factor is expressed also in other tissues such as breast, thyroid, colon, kidney, placenta and pancreas as well as heart, lymph node and spleen. Any lysates of these tissue could be used. And we do carry many of them in our catalog. However, since this antibody is specific to a phosphorylation site, you would need to check the biological conditions under which this site becomes phosphorylated. The datasheet states that the phospho-site is Serine 99. Upon checking the sequence in the UniPort database, I realized that the correct site is Ser103 (based on the sequence given: S-L-pS-E-M). There is no serine at residue 99. We will be correcting this oversight shortly. Here is the UniProt database link: http://www.uniprot.org/uniprot/P11831 where you will find also a reference for phosphorylation at S103: reference 6. "Identification of multiple SRF N-terminal phosphorylation sites affecting DNA binding properties." by Janknecht R. et al, EMBO J. 11:1045-1054(1992) [PubMed: 1547771] This might hopefully help you further in finding the conditions under which S103 is phosphorylated. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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