Recombinant Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (ab154825)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10684(B)] to Seryl-tRNA synthetase/SERS
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)]
See all Seryl-tRNA synthetase/SERS primary antibodies -
Description
Rabbit monoclonal [EPR10684(B)] to Seryl-tRNA synthetase/SERS -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Seryl-tRNA synthetase/SERS (N terminal). The exact sequence is proprietary.
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Positive control
- 293T and K562 cell lysates; Human pancreas tissue and Human thyroid gland carcinoma tissue; 293T cells and K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10684(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
(Heat to 98°C, allow to cool for 10-20 minutes) |
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ICC/IF |
1/100 - 1/250.
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Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 59 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) |
ICC/IF
1/100 - 1/250. |
Target
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Function
Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). -
Pathway
Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. -
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily. -
Domain
Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6301 Human
- Entrez Gene: 20226 Mouse
- Entrez Gene: 266975 Rat
- Omim: 607529 Human
- SwissProt: P49591 Human
- SwissProt: P26638 Mouse
- SwissProt: Q6P799 Rat
- Unigene: 531176 Human
see all -
Alternative names
- C78314 antibody
- cytoplasmic antibody
- EC 6.1.1.11 antibody
see all
Images
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All lanes : Anti-Seryl-tRNA synthetase/SERS antibody [EPR10684(B)] (ab154825) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 59 kDa -
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Seryl-tRNA synthetase/SERS with ab154825 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling Seryl-tRNA synthetase/SERS with ab154825 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunofluorescence analysis of 293T cells labeling Seryl-tRNA synthetase/SERS with ab154825 at 1/100 dilution.
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Intracellular flow cytometric analysis of permeabilized K562 cells labeling Seryl-tRNA synthetase/SERS with ab154825 at 1/100 dilution (red) or a negative Rabbit IgG (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab154825 has been referenced in 3 publications.
- Dai W et al. ASCT2 Is Involved in SARS-Mediated ß-Casein Synthesis of Bovine Mammary Epithelial Cells with Methionine Supply. J Agric Food Chem 68:13038-13045 (2020). PubMed: 31597423
- Dai WT et al. Seryl-tRNA synthetase-mediated essential amino acids regulate ß-casein synthesis via cell proliferation and mammalian target of rapamycin (mTOR) signaling pathway in bovine mammary epithelial cells. J Dairy Sci 101:10456-10468 (2018). PubMed: 30219419
- Xu L et al. Three distinct 3-methylcytidine (m3C) methyltransferases modify tRNA and mRNA in mice and humans. J Biol Chem 292:14695-14703 (2017). PubMed: 28655767