Recombinant
RabMAb

Recombinant Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (ab183025)

Overview

  • Product name

    Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685]
    See all Seryl-tRNA synthetase/SERS primary antibodies
  • Description

    Rabbit monoclonal [EPR10685] to Seryl-tRNA synthetase/SERS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human Seryl-tRNA synthetase/SERS aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: P49591

  • Positive control

    • HT29 , 293T, Jurkat, K562, PC12 and NIH 3T3 cell lysates; Human endometrium tissue.
  • General notes

     

     

     This product was previously labelled as Seryl-tRNA synthetase

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10685
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/40 - 1/60.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).

Target

  • Function

    Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).
  • Pathway

    Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.
  • Sequence similarities

    Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.
  • Domain

    Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • C78314 antibody
    • cytoplasmic antibody
    • EC 6.1.1.11 antibody
    • FLJ36399 antibody
    • sarS antibody
    • Sars1 antibody
    • Serine tRNA ligase 1, cytoplasmic antibody
    • Serine tRNA ligase antibody
    • Serine--tRNA ligase antibody
    • serine--tRNA ligase, cytoplasmic antibody
    • SerRS antibody
    • SERS antibody
    • Seryl tRNA Ser/Sec synthetase antibody
    • Seryl tRNA synthetase antibody
    • Seryl tRNA synthetase cytoplasmic antibody
    • Seryl-tRNA Ser/Sec synthetase antibody
    • Seryl-tRNA synthetase antibody
    • seryl-tRNA synthetase, cytoplasmic antibody
    • Seryl-tRNA(Ser/Sec) synthetase antibody
    • Strs antibody
    • SYSC_HUMAN antibody
    see all

Images

  • All lanes : Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (ab183025) at 1/5000 dilution

    Lane 1 : PC12 cell lysate
    Lane 2 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 59 kDa

  • Western blot analysis of immunoprecipitation pellet from Human K562 cell lysate immunoprecipitated using ab183025 at 1/50 dilution (lane 1) or a negative control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Seryl-tRNA synthetase/SERS with ab183025 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • All lanes : Anti-Seryl-tRNA synthetase/SERS antibody [EPR10685] (ab183025) at 1/20000 dilution

    Lane 1 : HT29 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 59 kDa

References

ab183025 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183025.
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