Key features and details
- Mouse polyclonal to SESN1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab67156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml. Requires purification with Protein A prior to ICC/IF.|
FunctionInvolved in the reduction of peroxiredoxins. May also be regulator of cellular growth.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the sestrin family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 1110002G11Rik antibody
- AU044290 antibody
- MGC118148 antibody
All lanes : Anti-SESN1 antibody (ab67156) at 1/500 dilution
Lane 1 : transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
Predicted band size: 57 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Immunofluorescence of ab67156 at 10 µg/ml on HeLa cells
ab67156 has been referenced in 3 publications.
- Chollat-Namy M et al. The pharmalogical reactivation of p53 function improves breast tumor cell lysis by granzyme B and NK cells through induction of autophagy. Cell Death Dis 10:695 (2019). PubMed: 31541080
- Pulito C et al. Metformin-induced ablation of microRNA 21-5p releases Sestrin-1 and CAB39L antitumoral activities. Cell Discov 3:17022 (2017). WB ; Human . PubMed: 28698800
- Pujals A et al. Constitutive autophagy contributes to resistance to TP53-mediated apoptosis in Epstein-Barr virus-positive latency III B-cell lymphoproliferations. Autophagy 11:2275-87 (2015). PubMed: 26565591