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Mouse polyclonal to SESN1
Full length protein, corresponding to amino acids 1-551 of Human SESN1
HeLa cells (IF/ICC)
Transfected 293T cell line (WB)
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein G purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
Use a concentration of 10 µg/ml. Requires purification with Protein A prior to ICC/IF.
Use a concentration of 5 µg/ml.
Involved in the reduction of peroxiredoxins. May also be regulator of cellular growth.
Belongs to the sestrin family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Western blot - Anti-SESN1 antibody (ab67156)
All lanes :
Anti-SESN1 antibody (ab67156) at 1/500 dilution
Lane 1 :
transfected 293T cell lysate
Lane 2 :
non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary All lanes :
Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
Predicted band size:
Observed band size:
75 kDa (
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-SESN1 antibody (ab67156)
Immunofluorescence of ab67156 at 10 µg/ml on HeLa cells
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SESN1 antibody (ab67156)
IHC image of ab67156 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica Bond
TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab67156, at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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