Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1930(2)] to SESN1
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-SESN1 antibody [EPR1930(2)]
See all SESN1 primary antibodies
DescriptionRabbit monoclonal [EPR1930(2)] to SESN1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SESN1 aa 100-200 (internal sequence). The exact sequence is proprietary.
- WB: Human heart, Ramos, K-562, JAR, HeLa, F9, C2C12, NIH/3T3, and Rat heart lysates. Flow Cyt: Daudi and permeabilized K562 cells,
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.08 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab134091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 57 kDa.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionInvolved in the reduction of peroxiredoxins. May also be regulator of cellular growth.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the sestrin family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 1110002G11Rik antibody
- AU044290 antibody
- MGC118148 antibody
All lanes : Anti-SESN1 antibody [EPR1930(2)] (ab134091) at 1/1000 dilution (Purified)
Lane 1 : Human heart lysates
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
Lane 3 : F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysates
Lane 4 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 6 : Rat heart lysates
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
The bands observed are consistent with what has been described in PMID: 29751091 and PMID: 29263116
Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells treated with 20 U/ml IFN alpha 1 for 24 hours (Red) / Untreated control (Green) labelling Anti-SESN1 with unpurified ab207414 at 1/600 dilution. Cells were fixed with 2% paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Black - isotype control, Rabbit monoclonal IgG (ab172730). Blue -unlabelled control, cells without incubation with primary and secondary antibodies.
Lanes 1 & 3-7 : Anti-SESN1 antibody [EPR1930(2)] (ab134091) at 1/1000 dilution ((unpurified))
Lane 2 : Anti-SESN1 antibody [EPR1930(2)] (ab134091) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : C2C12 cell lysate
Lane 5 : NIH3T3 cell lysate
Lane 6 : F9 cell lysate
Lane 7 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 57 kDa
Flow Cytometry analysis of permeabilized K562 cells labeling SESN1 with unpurified ab134091 at 1/100 dilution (red). Rabbit IgG (negative control) shown in green.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab134091 has been referenced in 4 publications.
- Lee T & Pelletier J Dependence of p53-deficient cells on the DHX9 DExH-box helicase. Oncotarget 8:30908-30921 (2017). PubMed: 28427210
- Wang X et al. Sestrin2 and sestrin3 suppress NK-92 cell-mediated cytotoxic activity on ovarian cancer cells through AMPK and mTORC1 signaling. Oncotarget 8:90132-90143 (2017). PubMed: 29163816
- Xue R et al. Sestrin 1 ameliorates cardiac hypertrophy via autophagy activation. J Cell Mol Med 21:1193-1205 (2017). PubMed: 28181410
- Kimball SR et al. Leucine induced dephosphorylation of Sestrin2 promotes mTORC1 activation. Cell Signal 28:896-906 (2016). PubMed: 27010498