Product nameAnti-SET antibody
See all SET primary antibodies
DescriptionRabbit polyclonal to SET
SpecificityReactive with I2aPP2A Does not react with I2bPP2A.
Tested applicationsSuitable for: IHC-P, IP, WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide, corresponding to amino acids 3-18 of Human I2 alpha PP2A.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab1183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 µg/ml.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionMultitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
Tissue specificityWidely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.
Involvement in diseaseNote=A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.
Sequence similaritiesBelongs to the nucleosome assembly protein (NAP) family.
DomainThe C-terminal acidic domain mediates the inhibition of histone acetyltransferases and is required for the DNA replication stimulatory activity.
modificationsIsoform 2 is phosphorylated on Ser-15 and Thr-23.
Isoform 2 is acetylated on Lys-11.
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated.
Cellular localizationCytoplasm > cytosol. Endoplasmic reticulum. Nucleus > nucleoplasm. In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. Following CTL attack, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium. The SET complex is associated with the endoplasmic reticulum.
- Information by UniProt
- 2PP2A antibody
- HLA DR associated protein II antibody
- HLA-DR-associated protein II antibody
Anti-SET antibody (ab1183) at 1/2000 dilution + HeLa cell lysate at 20 µg
Anti-rabbit IgG HRP-conjugate polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
Western blot of 10Western blot of 10 µg HEK 293 cell extracts with Anti- I2a (GLO150). The band corresponds to I2aPP2A (~ 39 kDa).
µg HEK 293 cell extracts with Anti- I2 α(GLO150). The band corresponds to I2 αPP2A (~ 39 kDa).
ICC/IF image of ab1183 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1183, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab35252 (4µg/ml) staining SET in human kidney cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear and cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
This product has been referenced in:
- Acuna-Hidalgo R et al. Overlapping SETBP1 gain-of-function mutations in Schinzel-Giedion syndrome and hematologic malignancies. PLoS Genet 13:e1006683 (2017). Read more (PubMed: 28346496) »
- Mody HR et al. SET contributes to the epithelial-mesenchymal transition of pancreatic cancer. Oncotarget 8:67966-67979 (2017). Read more (PubMed: 28978088) »