Anti-SET/TAF-I antibody (ab92872)
Key features and details
- Rabbit polyclonal to SET/TAF-I
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SET/TAF-I antibody
See all SET/TAF-I primary antibodies -
Description
Rabbit polyclonal to SET/TAF-I -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human SET/TAF-I aa 125-175.
Sequence:VTEFEDIKSG YRIDFYFDEN PYFENKVLSK EFHLNESGDP SSKSTEIKWK S
Database link: NP_001116293.1 -
Positive control
- WB: HeLa, 293T and NIH3T3 whole cell lysate, Lysate of the soluble fraction of HeLa or U20S cells. IHC-P: Human ovarian carcinoma and Mouse renal cell carcinoma tissue. IP: HeLa whole cell lysate.
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 33 kDa.
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IP |
Use at 3-5 µg/mg of lysate.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 33 kDa. |
IP
Use at 3-5 µg/mg of lysate. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. -
Tissue specificity
Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor. -
Involvement in disease
Note=A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN. -
Sequence similarities
Belongs to the nucleosome assembly protein (NAP) family. -
Domain
The C-terminal acidic domain mediates the inhibition of histone acetyltransferases and is required for the DNA replication stimulatory activity. -
Post-translational
modificationsIsoform 2 is phosphorylated on Ser-15 and Thr-23.
Isoform 2 is acetylated on Lys-11.
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated. -
Cellular localization
Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus > nucleoplasm. In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. Following CTL attack, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium. The SET complex is associated with the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 6418 Human
- Entrez Gene: 56086 Mouse
- Entrez Gene: 671392 Mouse
- Entrez Gene: 307947 Rat
- Omim: 600960 Human
- SwissProt: Q01105 Human
- SwissProt: Q9EQU5 Mouse
- SwissProt: Q63945 Rat
see all -
Alternative names
- 2PP2A antibody
- HLA DR associated protein II antibody
- HLA-DR-associated protein II antibody
see all
Images
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All lanes : Anti-SET/TAF-I antibody (ab92872) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Mouse NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 33 kDa
Exposure time: 30 secondsDetection: Chemiluminescence.
Lysates prepared using NETN lysis buffer.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue, labeling SET/TAF-I with ab92872 at 1/1000 (0.2 µg/mL) dilution. Detection: DAB.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue, labeling SET/TAF-I with ab92872 at 1/1000 (0.2 µg/mL) dilution. Detection: DAB.
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3µg ab92872 were used to immunoprecipitate SET/TAF-I from 1mg HeLa whole cell lysate. 20% IP was loaded and probed with ab92872 at 1µg/ml.
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Anti-SET/TAF-I antibody (ab92872) at 1/2000 dilution ((in 3% milk in PBST). Incubation was for 24 hours at 4°C.) + Lysate of the soluble fraction of HeLa or U20S cells at 30 µg
Secondary
An HRP polymer Goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: ~37,~40 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking Step: 5% Milk for 30 minutes at room temperature.
ab92872 recognises both isoforms of human SET/TAF-I.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab92872 has been referenced in 1 publication.
- Wang Y et al. Construction of SET overexpression vector and its effects on the proliferation and apoptosis of 293T cells. Mol Med Rep 13:4329-34 (2016). PubMed: 27035430