Overview

  • Product name
    Anti-SET antibody [EPR12972(B)]
    See all SET primary antibodies
  • Description
    Rabbit monoclonal [EPR12972(B)] to SET
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, Flow Cyt, WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SET aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q01105

  • Positive control
    • Jurkat, 293T, HepG2 and HeLa cell lysates; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab176567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
    • Tissue specificity
      Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.
    • Involvement in disease
      Note=A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.
    • Sequence similarities
      Belongs to the nucleosome assembly protein (NAP) family.
    • Domain
      The C-terminal acidic domain mediates the inhibition of histone acetyltransferases and is required for the DNA replication stimulatory activity.
    • Post-translational
      modifications
      Isoform 2 is phosphorylated on Ser-15 and Thr-23.
      Isoform 2 is acetylated on Lys-11.
      Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
      N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated.
    • Cellular localization
      Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus > nucleoplasm. In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. Following CTL attack, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium. The SET complex is associated with the endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2PP2A antibody
      • HLA DR associated protein II antibody
      • HLA-DR-associated protein II antibody
      • I 2PP2A antibody
      • I-2PP2A antibody
      • I2PP2A antibody
      • IGAAD antibody
      • Inhibitor of granzyme A activated DNase antibody
      • Inhibitor of granzyme A-activated DNase antibody
      • Inhibitor of GzmA-activated DNase antibody
      • inhibitor-2 of protein phosphatase-2A antibody
      • IPP2A2 antibody
      • PHAPII antibody
      • Phosphatase 2A inhibitor I2PP2A antibody
      • protein phosphatase type 2A inhibitor antibody
      • Protein SET antibody
      • Set antibody
      • SET nuclear oncogene antibody
      • SET translocation antibody
      • SET translocation (myeloid leukemia-associated) antibody
      • SET_HUMAN antibody
      • TAF I antibody
      • TAF IBETA antibody
      • TAF-I antibody
      • TAFI antibody
      • Template activating factor I antibody
      • Template-activating factor I antibody
      • Template-Activating Factor-I, chromatin remodelling factor antibody
      see all

    Images

    • All lanes : Anti-SET antibody [EPR12972(B)] (ab176567) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 33 kDa

    • Flow cytometry analysis of permeabilized Jurkat cells labeling SET (red) with ab176567 at a 1/10 dilution, or negative control rabbit IgG (green)

    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling SET with ab176567 at 1/10 dilution.

    References

    ab176567 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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