Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-SET3 antibody [EPR20101] (ab202434)

Overview

  • Product name

    Anti-SET3 antibody [EPR20101]
    See all SET3 primary antibodies
  • Description

    Rabbit monoclonal [EPR20101] to SET3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant fragment within Saccharomyces cerevisiae SET3 aa 1-300. The exact sequence is proprietary.
    Database link: P36124

  • Positive control

    • WB: Saccharomyces cerevisiae whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20101
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab202434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).

Images

  • Anti-SET3 antibody [EPR20101] (ab202434) at 1/2000 dilution + Saccharomyces cerevisiae whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 85 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-SET3 antibody [EPR20101] (ab202434) at 1/1000 dilution

    Lane 1 : Saccharomyces cerevisiae (WT) whole cell lysate
    Lane 2 : Saccharomyces cerevisiae (SET3 KO) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 85 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    KO strain (Y07046) was purchased from Euroscarf.

References

ab202434 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab202434.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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