Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-SET5 antibody [EPR20102] (ab202437)

Overview

  • Product name

    Anti-SET5 antibody [EPR20102]
    See all SET5 primary antibodies
  • Description

    Rabbit monoclonal [EPR20102] to SET5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant fragment within Saccharomyces cerevisiae SET5 aa 100-450. The exact sequence is proprietary.
    Database link: P38890

  • Positive control

    • WB: Saccharomyces cerevisiae whole cell lysate
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20102
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab202437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

Images

  • Anti-SET5 antibody [EPR20102] (ab202437) at 1/5000 dilution + Saccharomyces cerevisiae whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 61 kDa
    Observed band size: 61 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-SET5 antibody [EPR20102] (ab202437) at 1/5000 dilution

    Lane 1 : Saccharomyces cerevisiae (WT) whole cell lysate
    Lane 2 : Saccharomyces cerevisiae (SET5 KO) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 61 kDa
    Observed band size: 61 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Set5 KO strain (Y02901) was purchased from Euroscarf.

References

ab202437 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab202437.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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