Overview

  • Product name

    Anti-SET7 antibody [EPR5574]
    See all SET7 primary antibodies
  • Description

    Rabbit monoclonal [EPR5574] to SET7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SET7. The exact sequence is proprietary.
    Database link: Q8WTS6

  • Positive control

    • WB: HeLa, BxPC-3, Jurkat, MCF-7, C6, RAW264.7, PC-12, and NIH/3T3 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
IP 1/120.

For unpurified use at 1/10 - 1/100.

Target

  • Function

    Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.
  • Tissue specificity

    Widely expressed. Expressed in pancreatic islets.
  • Sequence similarities

    Belongs to the histone-lysine methyltransferase family. SET7 subfamily.
    Contains 3 MORN repeats.
    Contains 1 SET domain.
  • Domain

    The SET domain is necessary but not sufficient for histone methyltransferase activity.
  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ21193 antibody
    • H3 K4 HMTase antibody
    • H3-K4-HMTase SETD7 antibody
    • H4 lysine 4 specific antibody
    • Histone H3 K4 methyltransferase antibody
    • Histone H3 lysine 4 specific methyltransferase antibody
    • Histone H3-K4 methyltransferase SETD7 antibody
    • Histone H4 K4 methyltransferase antibody
    • Histone lysine N methyltransferase antibody
    • Histone lysine N methyltransferase H3 lysine 4 specific SET7 antibody
    • Histone-lysine N-methyltransferase SETD7 antibody
    • KIAA1717 antibody
    • KMT7 antibody
    • Lysine methyltransferase antibody
    • Lysine N-methyltransferase 7 antibody
    • OTTHUMP00000164543 antibody
    • OTTHUMP00000220049 antibody
    • SET 7 antibody
    • SET 7/9 antibody
    • SET 9 antibody
    • SET D7 antibody
    • SET domain containing (lysine methyltransferase) 7 antibody
    • SET domain containing protein 7 antibody
    • SET domain containing protein 8 antibody
    • SET domain-containing protein 7 antibody
    • SET7 antibody
    • SET7/9 antibody
    • SET9 antibody
    • Setd7 antibody
    • SETD7_HUMAN antibody
    see all

Images

  • All lanes : Anti-SET7 antibody [EPR5574] (ab124708) at 1/5000 dilution (purified)

    Lane 1 : PC-12 cell lysate
    Lane 2 : NIH/3T3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 41 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-SET7 antibody [EPR5574] (ab124708) at 1/5000 dilution (purified) + MCF-7 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 41 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-SET7 antibody [EPR5574] (ab124708) at 1/2000 dilution (purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 41 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-SET7 antibody [EPR5574] (ab124708) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : BxPC-3 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : MCF-7 cell lysate
    Lane 5 : C6 cell lysate
    Lane 6 : RAW264.7 cell lysate
    Lane 7 : PC-12 cell lysate
    Lane 8 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 41 kDa

  • ab124708 (purified) at 1/120 immunoprecipitating SET7 in Jurkat whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:

  • Song H  et al. Crosstalk between lysine methylation and phosphorylation of ATG16L1 dictates the apoptosis of hypoxia/reoxygenation-induced cardiomyocytes. Autophagy 14:825-844 (2018). Read more (PubMed: 29634390) »
  • Fulcoli FG  et al. Rebalancing gene haploinsufficiency in vivo by targeting chromatin. Nat Commun 7:11688 (2016). Read more (PubMed: 27256596) »
See all 2 Publications for this product

Customer reviews and Q&As

Question
Answer

Vielen Dank für Ihre Antwort.

Es tut mir leid, dass die Vorschläge das Problem nicht lösen konnten. Ich muss daraus schliessen, dass das Röhrchen welches Sie bekommen haben beschädigt ist. Es tut uns sehr leid. Wir senden Ihnen natürlich gerne einen Ersatz. Sie können wählen, ob Sie als Ersatz ein neues Röhrchen des ab14820 hätten, ein Röhrchen eines alternativen Antikörpers oder eine Gutschrift/Rückerstattung. Können Sie uns bitte hierzu auch die originale Bestellnummer des ab14820 geben? Falls Sie die Nummer nicht haben, können Sie uns das Datum der Bestellung sowie den Namen der Person die den ab14820 bestellt hat geben? Vielen Dank.

Leider haben wir zur Zeit jedoch keinen Setd7 Antikörper in unserem Katalog, der unseres Wissens nachauf Frosch oder Fischgewebe getestet wurde. Der ab124708 ist der einzige Antikörper gegen Setd7, der schon in IP und WB getestet wurde. Ich habe nun das Labor angefragt, ob die Immunogensequenz gewisse Homologie mit der Proteinsequenz von Setd7 von Fisch und Frosch hat. Ich werde mich melden sobald ich eine Antwort habe.

Unabhängig von der Ersatzsendung, können wir Ihnen dann auch ein Testangebot machen, falls Sie einen anderen Setd7 Antikörper von uns auf Fisch und Frosch testen wollen. Details eines solchen Angebots sind hier zu finden: https://www.abcam.com/collaborationdiscount

Ich freue mich wieder von Ihnen zu hören.

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