Product nameAnti-SET7 antibody [EPR5574] - BSA and Azide free
See all SET7 primary antibodies
DescriptionRabbit monoclonal [EPR5574] to SET7 - BSA and Azide free
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SET7. The exact sequence is proprietary.
Database link: Q8WTS6
Ab239434 is the carrier-free version of ab124708. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab239434 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Dissociation constant (KD)KD = 6.93 x 10 -10 M Learn more about KD
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
- HeLa nuclear extract lysate (ab14655)
- Jurkat nuclear extract lysate (ab14844)
- MCF7 nuclear extract lysate (ab14860)
- NIH 3T3 nuclear extract lysate (ab14874)
- PC12 nuclear extract lysate (ab14884)
- HeLa whole cell lysate (ab29545)
- MCF7 whole cell lysate (ab3871)
- NIH 3T3 whole cell lysate (ab7179)
- RAW 264.7 whole cell lysate (ab7187)
- Jurkat whole cell lysate (ab7899)
Our Abpromise guarantee covers the use of ab239434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.|
FunctionHistone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.
Tissue specificityWidely expressed. Expressed in pancreatic islets.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. SET7 subfamily.
Contains 3 MORN repeats.
Contains 1 SET domain.
DomainThe SET domain is necessary but not sufficient for histone methyltransferase activity.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- FLJ21193 antibody
- H3 K4 HMTase antibody
- H3-K4-HMTase SETD7 antibody
ab124708 (purified) at 1/120 immunoprecipitating SET7 in Jurkat whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) was used for detection at 1/1,500 dilution. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124708).
ab239434 has not yet been referenced specifically in any publications.