Overview

  • Product name

  • Description

    Rabbit polyclonal to SETD1B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human SETD1B aa 350-400.
    Database link: 23067

  • Positive control

    • HeLa cell lysate
  • General notes

    Protein previously labeled as KMT2G / hSET1B.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab113984 is affinity purified using an epitope specific to SETD1B immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

ab113984 has not performed satisfactorily when used for WB of SETD1B in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of SETD1B.

IP Use at 10 µg/mg of lysate.

Target

  • Function

    Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1A suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression. Specifically tri-methylates 'Lys-4' of histone H3 in vitro.
  • Sequence similarities

    Contains 1 post-SET domain.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 SET domain.
  • Cellular localization

    Nucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1A, suggesting that SETD1A and SET1B each bind to a unique set of target genes.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ20803 antibody
    • Histone-lysine N-methyltransferase SETD1B antibody
    • hSET1B antibody
    • KIAA1076 antibody
    • KMT2G antibody
    • Lysine N-methyltransferase 2G antibody
    • SET domain containing 1B antibody
    • SET domain-containing protein 1B antibody
    • SET1B antibody
    • SET1B_HUMAN antibody
    • SETD1B antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling SETD1B with ab113984 at 1/1000 (1µg/ml). Detection: DAB.

  • ab113984 used at 10 µg/mg of lysate staining SETD1B in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) by immunoprecipitation. For blotting immunoprecipitated SETD1B, ab113984 was used at 1 µg/ml.

    Detection: Chemiluminescence with an exposure time of 3 minutes.

References

This product has been referenced in:

  • Chen D  et al. High-level SETD1B gene expression is associated with unfavorable prognosis in hepatocellular carcinoma. Mol Med Rep 19:1587-1594 (2019). Read more (PubMed: 30628696) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Jun 19 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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