Key features and details
- Rabbit polyclonal to SETD1B
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SETD1B antibody
See all SETD1B primary antibodies
DescriptionRabbit polyclonal to SETD1B
Tested applicationsSuitable for: IHC-P, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Cow, Chimpanzee, Gorilla
Synthetic peptide corresponding to Human SETD1B aa 350-400.
Database link: 23067
- HeLa cell lysate
Protein previously labeled as KMT2G / hSET1B.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab113984 is affinity purified using an epitope specific to SETD1B immobilized on solid support.
Our Abpromise guarantee covers the use of ab113984 in the following tested applications.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at 10 µg/mg of lysate.|
FunctionHistone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1A suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression. Specifically tri-methylates 'Lys-4' of histone H3 in vitro.
Sequence similaritiesContains 1 post-SET domain.
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SET domain.
Cellular localizationNucleus speckle. Chromosome. Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1A, suggesting that SETD1A and SET1B each bind to a unique set of target genes.
- Information by UniProt
- FLJ20803 antibody
- Histone-lysine N-methyltransferase SETD1B antibody
- hSET1B antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling SETD1B with ab113984 at 1/1000 (1µg/ml). Detection: DAB.
ab113984 used at 10 µg/mg of lysate staining SETD1B in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) by immunoprecipitation. For blotting immunoprecipitated SETD1B, ab113984 was used at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.
ab113984 has been referenced in 3 publications.
- Lindner P et al. EMT transcription factor ZEB1 alters the epigenetic landscape of colorectal cancer cells. Cell Death Dis 11:147 (2020). PubMed: 32094334
- Yang P et al. SET domain containing 1B gene is mutated in primary hepatic neuroendocrine tumors. Int J Cancer 145:2986-2995 (2019). PubMed: 30977120
- Chen D et al. High-level SETD1B gene expression is associated with unfavorable prognosis in hepatocellular carcinoma. Mol Med Rep 19:1587-1594 (2019). PubMed: 30628696