Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab174662 was affinity purified using an epitope specific to SETD3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab174662 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 67 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionHistone methyltransferase that methylates 'Lys-36' of histone H3 (H3K36me). H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation.
Sequence similaritiesBelongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SETD3 subfamily.
Contains 1 SET domain.
- Information by UniProt
- C14orf154 antibody
- Chromosome 14 open reading frame 154 antibody
- DKFZp761E1415 antibody
Detection of SETD3 by Western Blot of Immunoprecipitate.
All lanes : Anti-SETD3 antibody (ab174662) at 0.100000001490116 µg/ml
Lane 1 : Jurkat whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : 293T whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 67 kDa
Exposure time: 30 seconds