Overview

  • Product name
  • Description
    Rabbit polyclonal to SETD3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SETD3 aa 544-594. The exact sequence is proprietary. NP_115609.2
    Sequence:

    KAKATENGLVNGENSIPNGTRSENESLNQESKRAVEDAKGSSSDSTAGVK E


    Database link: Q86TU7

  • Positive control
    • Whole cell lysates from Jurkat, HeLa and 293T cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab174662 was affinity purified using an epitope specific to SETD3 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 67 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Histone methyltransferase that methylates 'Lys-36' of histone H3 (H3K36me). H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation.
  • Sequence similarities
    Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SETD3 subfamily.
    Contains 1 SET domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C14orf154 antibody
    • Chromosome 14 open reading frame 154 antibody
    • DKFZp761E1415 antibody
    • FLJ23027 antibody
    • Histone lysine N methyltransferase setd3 antibody
    • Histone-lysine N-methyltransferase setd3 antibody
    • MGC87236 antibody
    • SET domain containing 3 antibody
    • SET domain containing protein 3 antibody
    • SET domain-containing protein 3 antibody
    • SETD 3 antibody
    • Setd3 antibody
    • SETD3_HUMAN antibody
    see all

Images

  • Detection of SETD3 by Western Blot of Immunoprecipitate.

  • All lanes : Anti-SETD3 antibody (ab174662) at 0.100000001490116 µg/ml

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : 293T whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 67 kDa


    Exposure time: 30 seconds

References

ab174662 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab174662.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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