Overview

  • Product name

  • Description

    Rabbit polyclonal to SET/TAF-I
  • Host species

    Rabbit
  • Specificity

    ab219389 may crossreact with P80349, rat Fuctinin-3. BLAST gives 100% homology.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human SET/TAF-I aa 13-28 (Cysteine residue).
    Sequence:

    Ac-LNSNHDGADETSEKEQc


    Database link: Q01105-2

  • Positive control

    • HeLa cells; Human small intestine tissue; Jurkat, HeLa, 293T, HL60, A431, L5178Y, WR19L, NIH 3T3, CHO and PC12 cell lysates.
  • General notes

     This product was previously labelled as SET

     

Properties

Applications

Our Abpromise guarantee covers the use of ab219389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/2000. Predicted molecular weight: 33 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
  • Tissue specificity

    Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.
  • Involvement in disease

    Note=A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.
  • Sequence similarities

    Belongs to the nucleosome assembly protein (NAP) family.
  • Domain

    The C-terminal acidic domain mediates the inhibition of histone acetyltransferases and is required for the DNA replication stimulatory activity.
  • Post-translational
    modifications

    Isoform 2 is phosphorylated on Ser-15 and Thr-23.
    Isoform 2 is acetylated on Lys-11.
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
    N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated.
  • Cellular localization

    Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus > nucleoplasm. In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. Following CTL attack, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium. The SET complex is associated with the endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2PP2A antibody
    • HLA DR associated protein II antibody
    • HLA-DR-associated protein II antibody
    • I 2PP2A antibody
    • I-2PP2A antibody
    • I2PP2A antibody
    • IGAAD antibody
    • Inhibitor of granzyme A activated DNase antibody
    • Inhibitor of granzyme A-activated DNase antibody
    • Inhibitor of GzmA-activated DNase antibody
    • inhibitor-2 of protein phosphatase-2A antibody
    • IPP2A2 antibody
    • PHAPII antibody
    • Phosphatase 2A inhibitor I2PP2A antibody
    • protein phosphatase type 2A inhibitor antibody
    • Protein SET antibody
    • Set antibody
    • SET nuclear oncogene antibody
    • SET translocation antibody
    • SET translocation (myeloid leukemia-associated) antibody
    • SET_HUMAN antibody
    • TAF I antibody
    • TAF IBETA antibody
    • TAF-I antibody
    • TAFI antibody
    • Template activating factor I antibody
    • Template-activating factor I antibody
    • Template-Activating Factor-I, chromatin remodelling factor antibody
    see all

Images

  • All lanes : Anti-SET/TAF-I antibody (ab219389) at 1/2000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HL60 cell lysate
    Lane 5 : A431 cell lysate
    Lane 6 : L5178Y cell lysate
    Lane 7 : WR19L cell lysate
    Lane 8 : NIH 3T3 cell lysate
    Lane 9 : CHO cell lysate
    Lane 10 : PC12 cell lysate

    Predicted band size: 33 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human small intestine tissue sections labeling SET/TAF-I with ab219389 at 5 µg/mL.

  • Immunofluorescence analysis of HeLa cells labeling SET/TAF-I with ab219389 at a 1/100 dilution.

References

ab219389 has not yet been referenced specifically in any publications.

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