Product nameAnti-SET/TAF-I antibody
See all SET/TAF-I primary antibodies
DescriptionRabbit polyclonal to SET/TAF-I
Specificityab219389 may crossreact with P80349, rat Fuctinin-3. BLAST gives 100% homology.
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human, Chinese hamster
- HeLa cells; Human small intestine tissue; Jurkat, HeLa, 293T, HL60, A431, L5178Y, WR19L, NIH 3T3, CHO and PC12 cell lysates.
This product was previously labelled as SET
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Constituents: 50% Glycerol, 50% PBS
Concentration information loading...
PurityProtein A purified
- HeLa whole cell lysate (ab150035)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- A431 whole cell lysate (ab30132)
- NIH 3T3 whole cell lysate (ab7179)
- Jurkat whole cell lysate (ab7899)
- A431 whole cell lysate (ab7909)
- HL60 whole cell lysate (ab7914)
- 293T whole cell lysate (ab95494)
Our Abpromise guarantee covers the use of ab219389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 33 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionMultitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
Tissue specificityWidely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.
Involvement in diseaseNote=A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.
Sequence similaritiesBelongs to the nucleosome assembly protein (NAP) family.
DomainThe C-terminal acidic domain mediates the inhibition of histone acetyltransferases and is required for the DNA replication stimulatory activity.
modificationsIsoform 2 is phosphorylated on Ser-15 and Thr-23.
Isoform 2 is acetylated on Lys-11.
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated.
Cellular localizationCytoplasm > cytosol. Endoplasmic reticulum. Nucleus > nucleoplasm. In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. Following CTL attack, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium. The SET complex is associated with the endoplasmic reticulum.
- Information by UniProt
- 2PP2A antibody
- HLA DR associated protein II antibody
- HLA-DR-associated protein II antibody
All lanes : Anti-SET/TAF-I antibody (ab219389) at 1/2000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HL60 cell lysate
Lane 5 : A431 cell lysate
Lane 6 : L5178Y cell lysate
Lane 7 : WR19L cell lysate
Lane 8 : NIH 3T3 cell lysate
Lane 9 : CHO cell lysate
Lane 10 : PC12 cell lysate
Predicted band size: 33 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human small intestine tissue sections labeling SET/TAF-I with ab219389 at 5 µg/mL.
Immunofluorescence analysis of HeLa cells labeling SET/TAF-I with ab219389 at a 1/100 dilution.
ab219389 has not yet been referenced specifically in any publications.