Overview

  • Product name

  • Description

    Rabbit polyclonal to SET/TAF-I
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Chicken, Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human SET/TAF-I aa 125-175.
    Sequence:

    VTEFEDIKSG YRIDFYFDEN PYFENKVLSK EFHLNESGDP SSKSTEIKWK S


    Database link: NP_001116293.1

  • Positive control

    • HeLa and 293T cell lysate
  • General notes

     This product was previously labelled as SET

     

Properties

Applications

Our Abpromise guarantee covers the use of ab92872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 33 kDa.
IP Use at 3-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
  • Tissue specificity

    Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.
  • Involvement in disease

    Note=A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.
  • Sequence similarities

    Belongs to the nucleosome assembly protein (NAP) family.
  • Domain

    The C-terminal acidic domain mediates the inhibition of histone acetyltransferases and is required for the DNA replication stimulatory activity.
  • Post-translational
    modifications

    Isoform 2 is phosphorylated on Ser-15 and Thr-23.
    Isoform 2 is acetylated on Lys-11.
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
    N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated.
  • Cellular localization

    Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus > nucleoplasm. In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. Following CTL attack, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium. The SET complex is associated with the endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2PP2A antibody
    • HLA DR associated protein II antibody
    • HLA-DR-associated protein II antibody
    • I 2PP2A antibody
    • I-2PP2A antibody
    • I2PP2A antibody
    • IGAAD antibody
    • Inhibitor of granzyme A activated DNase antibody
    • Inhibitor of granzyme A-activated DNase antibody
    • Inhibitor of GzmA-activated DNase antibody
    • inhibitor-2 of protein phosphatase-2A antibody
    • IPP2A2 antibody
    • PHAPII antibody
    • Phosphatase 2A inhibitor I2PP2A antibody
    • protein phosphatase type 2A inhibitor antibody
    • Protein SET antibody
    • Set antibody
    • SET nuclear oncogene antibody
    • SET translocation antibody
    • SET translocation (myeloid leukemia-associated) antibody
    • SET_HUMAN antibody
    • TAF I antibody
    • TAF IBETA antibody
    • TAF-I antibody
    • TAFI antibody
    • Template activating factor I antibody
    • Template-activating factor I antibody
    • Template-Activating Factor-I, chromatin remodelling factor antibody
    see all

Images

  • All lanes : Anti-SET/TAF-I antibody (ab92872) at 0.04 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 33 kDa


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SET/TAF-I with ab92872 at 1/400 (0.5µg/ml). A DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.

  • 3µg ab92872 were used to immunoprecipitate SET/TAF-I from 1mg HeLa whole cell lysate. 20% IP was loaded and probed with ab92872 at 1µg/ml.

  • Anti-SET/TAF-I antibody (ab92872) at 1/2000 dilution ((in 3% milk in PBST). Incubation was for 24 hours at 4°C.) + Lysate of the soluble fraction of HeLa or U20S cells at 30 µg

    Secondary
    An HRP polymer Goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 33 kDa
    Observed band size: ~37,~40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking Step: 5% Milk for 30 minutes at room temperature.

    ab92872 recognises both isoforms of human SET/TAF-I.

    See Abreview

References

ab92872 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Chicken Cell lysate - other (Chicken lymphoblasts DT40)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
Chicken lymphoblasts DT40
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Jul 19 2010

Application
Western blot
Sample
Human Cell lysate - other (Soluble Fraction (HeLa or U2OS))
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
Soluble Fraction (HeLa or U2OS)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Jun 16 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up