Recombinant Anti-SET/TAF-I antibody [EPR12972(B)] (ab176567)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12972(B)] to SET/TAF-I
- Suitable for: IP, Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SET/TAF-I antibody [EPR12972(B)]
See all SET/TAF-I primary antibodies -
Description
Rabbit monoclonal [EPR12972(B)] to SET/TAF-I -
Host species
Rabbit -
Tested applications
Suitable for: IP, Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human SET/TAF-I aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q01105 -
Positive control
- Jurkat, 293T, HepG2 and HeLa whole cell lysate (ab150035); Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12972(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176567 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/10 - 1/100.
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
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Notes |
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IP
1/10 - 1/100. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 33 kDa. |
Target
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Function
Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. -
Tissue specificity
Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor. -
Involvement in disease
Note=A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN. -
Sequence similarities
Belongs to the nucleosome assembly protein (NAP) family. -
Domain
The C-terminal acidic domain mediates the inhibition of histone acetyltransferases and is required for the DNA replication stimulatory activity. -
Post-translational
modificationsIsoform 2 is phosphorylated on Ser-15 and Thr-23.
Isoform 2 is acetylated on Lys-11.
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated. -
Cellular localization
Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus > nucleoplasm. In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. Following CTL attack, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium. The SET complex is associated with the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 6418 Human
- Entrez Gene: 56086 Mouse
- Entrez Gene: 671392 Mouse
- Entrez Gene: 307947 Rat
- Omim: 600960 Human
- SwissProt: Q01105 Human
- SwissProt: Q9EQU5 Mouse
- SwissProt: Q63945 Rat
see all -
Alternative names
- 2PP2A antibody
- HLA DR associated protein II antibody
- HLA-DR-associated protein II antibody
see all
Images
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All lanes : Anti-SET/TAF-I antibody [EPR12972(B)] (ab176567) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa -
Intracellular Flow Cytometry analysis of permeabilized Jurkat cells labeling SET/TAF-I (red) with ab176567 at a 1/10 dilution, or negative control rabbit IgG (green)
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Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling SET/TAF-I with ab176567 at 1/10 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab176567 has been referenced in 2 publications.
- Frohner IE et al. PP2AC Phospho-Tyr307 Antibodies Are Not Specific for this Modification but Are Sensitive to Other PP2AC Modifications Including Leu309 Methylation. Cell Rep 30:3171-3182.e6 (2020). PubMed: 32130916
- Apostolidis SA et al. Phosphatase PP2A is requisite for the function of regulatory T cells. Nat Immunol 17:556-64 (2016). PubMed: 26974206