Recombinant
RabMAb

Recombinant Anti-SET/TAF-I antibody [EPR12973] (Phycoerythrin) (ab237445)

Overview

  • Product name

    Anti-SET/TAF-I antibody [EPR12973] (Phycoerythrin)
    See all SET/TAF-I primary antibodies
  • Description

    Rabbit monoclonal [EPR12973] to SET/TAF-I (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SET aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q01105

  • Positive control

    • FC: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab237445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2500.

Target

  • Function

    Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
  • Tissue specificity

    Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.
  • Involvement in disease

    Note=A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.
  • Sequence similarities

    Belongs to the nucleosome assembly protein (NAP) family.
  • Domain

    The C-terminal acidic domain mediates the inhibition of histone acetyltransferases and is required for the DNA replication stimulatory activity.
  • Post-translational
    modifications

    Isoform 2 is phosphorylated on Ser-15 and Thr-23.
    Isoform 2 is acetylated on Lys-11.
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
    N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated.
  • Cellular localization

    Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus > nucleoplasm. In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. Following CTL attack, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium. The SET complex is associated with the endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2PP2A antibody
    • HLA DR associated protein II antibody
    • HLA-DR-associated protein II antibody
    • I 2PP2A antibody
    • I-2PP2A antibody
    • I2PP2A antibody
    • IGAAD antibody
    • Inhibitor of granzyme A activated DNase antibody
    • Inhibitor of granzyme A-activated DNase antibody
    • Inhibitor of GzmA-activated DNase antibody
    • inhibitor-2 of protein phosphatase-2A antibody
    • IPP2A2 antibody
    • PHAPII antibody
    • Phosphatase 2A inhibitor I2PP2A antibody
    • protein phosphatase type 2A inhibitor antibody
    • Protein SET antibody
    • Set antibody
    • SET nuclear oncogene antibody
    • SET translocation antibody
    • SET translocation (myeloid leukemia-associated) antibody
    • SET_HUMAN antibody
    • TAF I antibody
    • TAF IBETA antibody
    • TAF-I antibody
    • TAFI antibody
    • Template activating factor I antibody
    • Template-activating factor I antibody
    • Template-Activating Factor-I, chromatin remodelling factor antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab237445 (red line).

    The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal Goat serum to block non-specific protein-protein interactions followed by the antibody (ab237445) (1x 106 in 100 µl at 0.2 µg/ml (1/2500 dilution)) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab237445 has not yet been referenced specifically in any publications.

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