Recombinant
RabMAb

Recombinant Anti-Sex Hormone Binding Globulin/SHBG antibody [EPR21758] (ab219575)

Overview

  • Product name

    Anti-Sex Hormone Binding Globulin/SHBG antibody [EPR21758]
    See all Sex Hormone Binding Globulin/SHBG primary antibodies
  • Description

    Rabbit monoclonal [EPR21758] to Sex Hormone Binding Globulin/SHBG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Sex Hormone Binding Globulin/SHBG aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P04278

  • Positive control

    • WB: Human serum and testis lysates; HepG2 cell supernatant.
  • General notes

    Previously labelled as Sex Hormone Binding Globulin. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21758
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab219575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

Target

  • Function

    Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
  • Tissue specificity

    Isoform 1 and isoform 2 are present in liver and testis.
  • Sequence similarities

    Contains 2 laminin G-like domains.
  • Cellular localization

    Secreted. In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABP antibody
    • Androgen binding protein antibody
    • SBP antibody
    • Sex hormone-binding globulin antibody
    • Sex steroid binding protein antibody
    • Sex steroid-binding protein antibody
    • SHBG antibody
    • SHBG_HUMAN antibody
    • TeBG antibody
    • Testis specific androgen binding protein antibody
    • Testis-specific androgen-binding protein antibody
    • Testosterone binding beta globulin antibody
    • Testosterone estradiol binding globulin antibody
    • Testosterone estrogen binding globulin antibody
    • Testosterone-estradiol-binding globulin antibody
    • Testosterone-estrogen-binding globulin antibody
    see all

Images

  • All lanes : Anti-Sex Hormone Binding Globulin/SHBG antibody [EPR21758] (ab219575) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) supernatant
    Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell) supernatant

    Lysates/proteins at 10 µl per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/500 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 3 minutes


    Blocking/ Dilution buffer and concentration: 5% NFDM/TBST.

    Negative control: MCF-7 (PMID:8545857)

  • All lanes : Anti-Sex Hormone Binding Globulin/SHBG antibody [EPR21758] (ab219575) at 1/1000 dilution

    Lane 1 : Human serum at 20 µg
    Lane 2 : Human testis lysate at 10 µg

    Secondary
    Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 3 minutes


    Blocking/ Dilution buffer and concentration: 5% NFDM/TBST.

References

ab219575 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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