Recombinant
RabMAb

Recombinant Anti-Sex Hormone Binding Globulin/SHBG antibody [EPR21758] - BSA and Azide free (ab233703)

Overview

  • Product name

    Anti-Sex Hormone Binding Globulin/SHBG antibody [EPR21758] - BSA and Azide free
    See all Sex Hormone Binding Globulin/SHBG primary antibodies
  • Description

    Rabbit monoclonal [EPR21758] to Sex Hormone Binding Globulin/SHBG - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Sex Hormone Binding Globulin/SHBG aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P04278

  • Positive control

    • WB: Human serum and testis lysates.
  • General notes

    ab233703 is the carrier-free version of ab219575 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab233703 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as Sex Hormone Binding Globulin.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab233703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

Target

  • Function

    Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
  • Tissue specificity

    Isoform 1 and isoform 2 are present in liver and testis.
  • Sequence similarities

    Contains 2 laminin G-like domains.
  • Cellular localization

    Secreted. In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABP antibody
    • Androgen binding protein antibody
    • SBP antibody
    • Sex hormone-binding globulin antibody
    • Sex steroid binding protein antibody
    • Sex steroid-binding protein antibody
    • SHBG antibody
    • SHBG_HUMAN antibody
    • TeBG antibody
    • Testis specific androgen binding protein antibody
    • Testis-specific androgen-binding protein antibody
    • Testosterone binding beta globulin antibody
    • Testosterone estradiol binding globulin antibody
    • Testosterone estrogen binding globulin antibody
    • Testosterone-estradiol-binding globulin antibody
    • Testosterone-estrogen-binding globulin antibody
    see all

Images

  • All lanes : Anti-Sex Hormone Binding Globulin/SHBG antibody [EPR21758] (ab219575) at 1/1000 dilution

    Lane 1 : Human serum at 20 µg
    Lane 2 : Human testis lysate at 10 µg

    Secondary
    Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa


    Exposure time: 3 minutes


    Blocking/ Dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219575).

References

ab233703 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab233703.
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