Overview

  • Product name

    Anti-SF3A1 antibody [EPR7666]
    See all SF3A1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7666] to SF3A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human SF3A1 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • HeLa, lymph node and K562 cell lysates; Human breast tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7666
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 89 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Contains 2 SURP motif repeats.
      Contains 1 ubiquitin-like domain.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Pre mRNA processing 21 antibody
      • Pre mRNA splicing factor SF3a antibody
      • PRP21 antibody
      • PRPF21 antibody
      • SAP 114 antibody
      • SAP114 antibody
      • sf3a1 antibody
      • SF3A1_HUMAN antibody
      • SF3a120 antibody
      • Spliceosome associated protein 114 antibody
      • Spliceosome-associated protein 114 antibody
      • Splicing factor 3 subunit 1 antibody
      • Splicing factor 3a subunit 1 120kDa antibody
      • Splicing factor 3A subunit 1 antibody
      see all

    Images

    • ab128868, at a 1/50 dilution, staining SF3A1 in paraffin embedded Human breast tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • All lanes : Anti-SF3A1 antibody [EPR7666] (ab128868) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Lymph node lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 89 kDa

    References

    ab128868 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for contacting us.

    We have published safety datasheets for these products on the website. These will be available soon. Please download these from our website.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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