1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/50 - 1/250.
SF3A3 is subunit 3 of the splicing factor SF3A. Splicing factor 3a is a heterotrimer consisting of subunits 1, 2 and 3, and is necessary for the conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. SF3A interacts with the pre-mRNA branch site early during spliceosome formation.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling SF3A3 with ab176581 at 1µg/ml.
Western blot - Anti-SF3A3 antibody (ab176581)
All lanes : Anti-SF3A3 antibody (ab176581) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Detection of SF3A3 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176581 at 3 µg/mg lysate for IP (Lane 1). For WB detection ab176581 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.